Transcriptomics,Genomics

Dataset Information

45

NHDF Cells (Normal Human Dermal Fibroblasts): Control (unstimulated fibroblasts) vs. Borrelia infected cells


ABSTRACT: Transcriptional profiling of NHDF Cells comparing control untreated fibroblasts with fibroblasts coincubated with three different species of the Borrelia burgdorferi sensu lato group. Overall design: Four-conditions experiment (unstimulated NHDF; NHDF stimulated by Borrelia burgdorferi sensu stricto strain IBS19; NHDF stimulated by Borrelia garinii strain IBS6; NHDF stimulated by Borrelia garinii strain IBS17). There is 6 Biological replicates for each condition (i.e. 6 different batches of NHDF cells from 6 independent human donor), independently grown and harvested.

INSTRUMENT(S): Illumina HumanHT-12 V4.0 expression beadchip

SUBMITTER: Frédéric Schramm  

PROVIDER: GSE77058 | GEO | 2016-05-22

SECONDARY ACCESSION(S): PRJNA309374

REPOSITORIES: GEO

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Publications

Homogeneous Inflammatory Gene Profiles Induced in Human Dermal Fibroblasts in Response to the Three Main Species of Borrelia burgdorferi sensu lato.

Meddeb Mariam M   Carpentier Wassila W   Cagnard Nicolas N   Nadaud Sophie S   Grillon Antoine A   Barthel Cathy C   De Martino Sylvie Josiane SJ   Jaulhac Benoît B   Boulanger Nathalie N   Schramm Frédéric F  

PloS one 20161005 10


In Lyme borreliosis, the skin is the key site for bacterial inoculation by the infected tick and for cutaneous manifestations. We previously showed that different strains of Borrelia burgdorferi sensu stricto isolated from tick and from different clinical stages of the Lyme borreliosis (erythema migrans, and acrodermatitis chronica atrophicans) elicited a very similar transcriptional response in normal human dermal fibroblasts. In this study, using whole transcriptome microarray chips, we aimed  ...[more]

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