Transcriptomics,Genomics

Dataset Information

156

Sequencing of synthetic small RNAs hsa-miR-34b-5p, hsa-miR-449c-5p and their isomir variant


ABSTRACT: The aim of this small RNA Seq is to determine if the sequence reads observed for hsa-miR-34b-5p and hsa-miR-449c-5p represent library artifacts or sequencing artifacts Overall design: Each mature miRNA isomer has been sequenced alone in a complex mixture of RNA. The two forms of hsa-miR-34b-5p and hsa-miR-449c-5p were sequenced with a 1:1 ratio (mix)

INSTRUMENT(S): AB 5500xl-W Genetic Analysis System (Homo sapiens)

SUBMITTER: Kevin Lebrigand 

PROVIDER: GSE77249 | GEO | 2017-06-12

SECONDARY ACCESSION(S): PRJNA309857

REPOSITORIES: GEO

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Publications


miR-34/449 microRNAs are conserved regulators of multiciliated cell differentiation. Here, we evidence and characterize expression of two isomiR variant sequences from the miR-34/449 family in human airway epithelial cells. These isomiRs differ from their canonical counterparts miR-34b and miR-449c by one supplemental uridine at their 5'-end, leading to a one-base shift in their seed region. Overexpression of canonical miR-34/449 or 5'-isomiR-34/449 induces distinct gene expression profiles and  ...[more]

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