Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiR-seq to define mature miRNAs in human HEK293T cells that are targeted for degradation by the endonuclease Tudor-SN (i.e. by TumiD).

ABSTRACT: We report that human Tudor-SN degrades particular miRNAs that contain CA or UA dinucleotides located more than five nucleotides from miRNA ends.

ORGANISM(S): Homo sapiens

PROVIDER: GSE77484 | GEO | 2017/05/25

SECONDARY ACCESSION(S): PRJNA310615

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

UPF1 helicase promotes Tudor-SN-mediated miRNA decay

Project description:The helicase activity of UPF1 remodels miRNPs so as to render miRNAs susceptible to Tudor-SN (TSN)-mediated miRNA decay

2017-08-22 | GSE100068 | GEO

Gene expression in Tudor-SN knockdown Drosophila in normoxic and hypoxic conditions

Project description:The RNA-binding protein Snd1/Tudor-SN can regulate gene expression through various mechanisms and is suggested to function in stress responses. Here we have analyzed mRNA expression in Tudor-SN knockdown and control flies in hypoxic and normoxic conditions.

2020-01-28 | GSE144308 | GEO

Transcriptome changes caused by Tudor-SN mutations in rice seed

Project description:One OsTudor-SN mutant, EM1084, carrying E503K point mutation and one associated WT-OsTSN complemented transgenic (pHL8042/EM1084 ) were applied in this study. EM1084 was obtained by TILLING studies (http://www.shigen.nig.ac.jp/rice/oryzabase). The E503K mutation site in EM1084 lies within the SN3 domain, while the EM1084 complemented transgenic plant was complemented with the full-length WT OsTSN gene driven by native OsTSN promoter. This mutation results in affecting storage protein mRNA localization and, in turn, with a strong reduction in grain weight and storage protein accumulation. Transcriptome analysis on the dehulled 10-14 days old developing seeds from wild type, EM1084 and pHL8042/EM1084 indicates that partial loss of Tudor-SN function caused the differential expression of storage protein genes and relevant genes involved in several essential biological processes during rice development.

2019-06-07 | GSE132303 | GEO

Transcriptome changes caused by Tudor-SN mutations in rice seed

Project description:Transcriptome changes caused by Tudor-SN mutations in rice seed

| PRJNA547574 | ENA

Gene expression in Tudor-SN knockdown Drosophila in normoxic and hypoxic conditions

Project description:Gene expression in Tudor-SN knockdown Drosophila in normoxic and hypoxic conditions

| PRJNA603345 | ENA

Hydrogenobaculum sp. SN strain:SN

Project description:Hydrogenobaculum sp. SN genome sequencing

| PRJNA39709 | ENA

Transcriptomic analysis of tudor[1] mutant Drosophila brain and ovaries

Project description:In Drosophila, Tudor protein and its germline partners, Piwi proteins, are expressed in the brain. However, the potential significance of Tudor in neurobiology has not been explored. Here, we test a hypothesis that Tudor is an essential regulator of post-transcriptional gene expression in the brain where it controls levels of certain RNAs required for brain functions. Specifically, transcriptome of tudor mutant brains is compared with that of wild-type brains using next-generation sequencing (RNA-Seq). The hypothesis that Tudor regulates the same genes in both brain and germline is tested by comparing the transcriptomes from tudor mutant brains and ovaries. This research aims at providing innovative outcomes which may reveal exciting commonalities between the germline and brain and may contribute to our understanding of neurodegenerative disorders and the mechanisms of learning and memory.

2020-11-27 | GSE149745 | GEO

Transcriptome analysis of mouse NSN and SN oocytes classified by Fibrillarin-GFP.

Project description:We selected NSN and SN GV oocytes based on FBL-GFP localization, and performed transcriptome profiling of single NSN and SN GV oocytes, and MII oocytes in vitro matured from NSN and SN GV oocytes.

2021-03-27 | GSE163935 | GEO

H3K14ac is linked to methylation of H3K9 by the Tudor domains of SETDB1

Project description:Mononucleosomes were isolated from murine ES cells and precipitated with the recombinant SETDB1 Triple Tudor Domain (3TD), recombinant SETDB1 Triple Tudor domain Y268A mutant, anti-H3K9me2 (Abcam, ab1220), or anti-H3K9me1 (Abcam, ab8896, Lot GR185298-1) antibodies.

2018-07-30 | E-MTAB-4982 | biostudies-arrayexpress

Role of the Tudor-knot domain of the TIP60 lysine acetyltransferase [RNA-seq]

Project description:The histone lysine acetyltransferase TIP60 is the main enzyme that catalyzes histone H4 acetylation in cells. Domains on TIP60 regulate its enzymatic activity from different aspects. Here we use a CRISPR-Cas9 tiling screen to scan for essential domains on TIP60 protein and found that the Tudor-knot domain is essential for cell survival and intercellular H4 acetylation. We performed in-vitro biochemical assays and demonstrated Tudor-knot domain is not a histone reader. And deficiency of the Tudor-knot domain has mild effects on TIP60 intracellular localization, as well as the TIP60 complex’s constitution. But Tudor-knot deficiency significantly reduces TIP60 HAT activity both in vivo and in vitro. By comparing the catalytic efficiency of nucleosome substrate and histone octamer substrate, as well as TIP60 protein alone or TIP60 complex, we found the nucleosomal structure and other TIP60 complex components are required for Tudor-knot relative HAT activity regulation. We propose that the Tudor-knot domain function to increase nucleosome accessibility. Finally, we show that the Tudor-knot domain is required for TIP60-dependent transcription regulation. Altogether, our study reveals a mechanism that the Tudor-knot domain that regulates TIP60-dependent transcription through the regulation of TIP60 substrate catalytic efficiency.

2023-12-24 | GSE245115 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data