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Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

ABSTRACT: We describe a protocol for freezing neuronal cells that is compatible with ATAC-Seq, producing results that compare well with those generated from fresh cells. We developed our protocol on a disease-relevant cell type, namely motor neurons differentiated from induced pluripotent stem cells from a patient affected by spinal muscular atrophy. We found that while flash-frozen motor neurons are not suitable for ATAC-Seq, the assay is successful with slow-cooled cryopreserved samples. Using this method, we were able to isolate high quality, intact nuclei, and we verified that epigenetic results from fresh and cryopreserved motor neurons agree quantitatively.

ORGANISM(S): Homo sapiens

PROVIDER: GSE78036 | GEO | 2016/05/05

SECONDARY ACCESSION(S): PRJNA312393

REPOSITORIES: GEO

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