Dataset Information


Gene expression signature after abrupt glucose starvation in Lactococcus lactis

ABSTRACT: Stressed samples: Strain MG1363 was exposed to abrupt glucose depletion in SA medium after balanced growth. Reference sample: Strain MG1363 was grown balanced in SA medium containing 1% glucose Overall design: Samples were taken from reference culture at OD450=0.15, and from glucose starved cultures 10 and 60 min after depletion.

INSTRUMENT(S): Agilent-031795 L.lactis_and_2_phages

SUBMITTER: Mogens Kilstrup  

PROVIDER: GSE78882 | GEO | 2016-03-04



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Ribosomal dimerization factor YfiA is the major protein synthesized after abrupt glucose depletion in Lactococcus lactis.

Breüner Anne A   Frees Dorte D   Varmanen Pekka P   Boguta Anna Monika AM   Hammer Karin K   Martinussen Jan J   Kilstrup Mogens M  

Microbiology (Reading, England) 20160823 10

We analysed the response of the model bacterium Lactococcus lactis to abrupt depletion of glucose after several generations of exponential growth. Glucose depletion resulted in a drastic drop in the energy charge accompanied by an extremely low GTP level and an almost total arrest of protein synthesis. Strikingly, the cell prioritized the continued synthesis of a few proteins, of which the ribosomal dimerization factor YfiA was the most highly expressed. Transcriptome analysis showed no immediat  ...[more]

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