Genomics

Dataset Information

35

Novel cell lines from mouse epiblast share defining features with human embryonic stem cells


ABSTRACT: The application of human embryonic stem (ES) cells has an inherent reliance on understanding the starting cell population. Human ES cells differ from mouse ES cells and the specific embryonic origin of both cell types is unclear. Previous work suggested that mouse ES cells could only be obtained from the embryo prior to implantation in the uterus. Here we show that cell lines can be derived from the epiblast, a tissue of the post-implantation embryo that generates the embryo proper. These cells, which we refer to as EpiSCs (post-implantation epiblast-derived stem cells), express transcription factors known to regulate pluripotency, maintain their genomic integrity, and robustly differentiate into the major somatic cell types as well as primordial germ cells (PGCs). The post-ES cell lines are distinct from mouse ES cells in their epigenetic state and the signals controlling their differentiation. Furthermore, post-ES and human ES cells share patterns of gene expression and signalling responses that normally function in the epiblast. These results show that epiblast cells can be maintained as stable cell lines and interrogated to understand how pluripotent cells generate distinct fates during early development. This SuperSeries is composed of the SubSeries listed below. Overall design: Refer to individual Series.

REANALYSED by: GSE63844

INSTRUMENT(S): Sentrix Human-6 Expression BeadChip

SUBMITTER: Paul Tesar  

PROVIDER: GSE7902 | GEO | 2007-06-27

SECONDARY ACCESSION(S): GSE7865 GSE7866PRJNA99953 GSE7900

REPOSITORIES: GEO

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New cell lines from mouse epiblast share defining features with human embryonic stem cells.

Tesar Paul J PJ   Chenoweth Josh G JG   Brook Frances A FA   Davies Timothy J TJ   Evans Edward P EP   Mack David L DL   Gardner Richard L RL   McKay Ronald D G RD  

Nature 20070627 7150


The application of human embryonic stem (ES) cells in medicine and biology has an inherent reliance on understanding the starting cell population. Human ES cells differ from mouse ES cells and the specific embryonic origin of both cell types is unclear. Previous work suggested that mouse ES cells could only be obtained from the embryo before implantation in the uterus. Here we show that cell lines can be derived from the epiblast, a tissue of the post-implantation embryo that generates the embry  ...[more]

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