Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global profiling of microRNAs in peripheral blood and synovial fluid mononuclear cells of patients with Enthesitis Related Arthritis

ABSTRACT: Enthesitis related arthritis (ERA) is the most common category of juvenile idiopathic arthritis in Asia. MicroRNA dysregulation has been shown in chronic arthritides however there is no data in ERA. MicroRNA dysregulation in ERA may help understand the pathogenesis of disease. Thus we determined the difference in global miRNA expression in peripheral blood mononuclear cells (PBMCs) of patients with ERA (n=8) as compared to healthy controls (HC) (n=8) and further to understand the changes at the site of inflammation, the PBMCs profile was compared with the synovial fluid mononuclear cells (SFMCs) (n=8) of patients with ERA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE79481 | GEO | 2017/10/30

SECONDARY ACCESSION(S): PRJNA315968

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Single-cell RNA-seq of CD8 T cells sorted from one sample of seropositive rheumatoid arthritis synovial fluid

Project description:We used FACS to sort CD8 T cells from synovial fluid mononuclear cells from one patient with seropositive rheumatoid arthritis

2022-05-08 | GSE202366 | GEO

Proteome characterization of synovial fluid polarized healthy monocytes

Project description:This project involves proteome characterization of healthy monocytes polarized (activated) with synovial fluid (SF) versus serum from patients with juvenile idiopathic arthritis (JIA). The objective is to investigate the influence of SF on monocytes at the proteome level, using serum polarized monocytes as a control. Monocytes were isolated from peripheral blood mononuclear cells of healthy donors following negative selection and were subsequently polarized overnight with 20% of a pool of either serum or SF. They were subsequently detached and used for proteome analysis.

2023-07-20 | PXD033983 | Pride

Characterisation of peripheral blood mononuclear cell microRNA in Early Onset Psoriatic Arthritis

Project description:The expression of 723 mature miRNAs in peripheral blood mononuclear cells of patients with early, active psoriatic arthritis ,early-rheumatoid arthritis (ERA) (both groups treatment naïve) and of healthy controls (HC) was analysed using a miRNA microarray.

2016-12-01 | E-MTAB-3956 | biostudies-arrayexpress

Bulk RNA-seq of human synovial fluid CD8 T cells sorted by Granzyme B vs Granzyme K expression

Project description:Cryopreserved synovial fluid mononuclear cells from three patients with seropositive rheumatoid arthritis were thawed and stained for surface antibodies, then fixed and stained for intracellular granzyme K and granzyme B. CD8 T cells with the following expression patterns were then isolated by FACS.

2022-05-08 | GSE202365 | GEO

Gene Expression Profiling in Peripheral Blood in Untreated New Onset Systemic Juvenile Idiopathic Arthritis

Project description:Systemic Juvenile Idiopathic Arthritis (sJIA) has been strongly associated with macrophage activation syndrome (MAS). To better understand the pathogenesid of sJIA and to facilitate the search for MAS biomarkers, we examine gene expression profiles in untreated new onset sJIA. 17 new onset sJIA patients were included in the study. 5 of the 17 patients showed evidence of subclinical MAS and 2 eventually developed overt MAS. Keywords: disease versus control Peripheral blood mononuclear cells (PBMCs) were separated using a Ficoll gradient from the 17 new onset sJIA patients and 30 normal control. RNA was extracted from the PBMCS and subsequently hybridized to Affymetrix microarrays

2007-11-01 | E-GEOD-7753 | biostudies-arrayexpress

10x single cell gene expression and V(D)J immune profiling of T cells from the blood, synovial fluid and synovial tissue in psoriatic arthritis patients

Project description:This study shows the pronounced expansion of CD8 T cell clones at sites of active inflammation within the joints of psoriatic arthritis (PsA) patients compared to blood, and characterises the gene expression of expanded clones using droplet based single cell RNA sequencing. Mononuclear cells were separated from freshly acquired paired peripheral blood and synovial fluid samples from 3 PsA patients using density gradient separation, then enriched for CD4 and CD8 T cells using FACS. Additionally, CD45+ leukocytes were enriched by FACS from the cryopreserved synovial tissue of 2 further PsA patients. All cells were then processed using a 10x Chromium Controller and sequenced by Illumina HiSeq 4000 (peripheral blood / synovial fluid) or NovaSeq with S2 flowcell (synovial tissue).

2020-09-07 | E-MTAB-9492 | biostudies-arrayexpress

Synovial fluid proteome in rheumatoid arthritis

Project description:Rheumatoid arthritis (RA) is a chronic autoinflammatory disorder that affects small joints. Despite intense efforts, there isno definitive marker yet for early diagnosis RA and for monitoring the progression of this disease. We sought to catalog the proteins present in the synovial fluid of patients with rheumatoid arthritis. To identify lower abundance proteins, we undertook two approaches – we depleted the abundant proteins using a multiple affinity removal system (MARS14) column and we enriched glycoproteins using a lectin affinity column. The peptides were analyzed by LC-MS/MS on a high resolution Fourier transform mass spectrometer.

2016-06-13 | PXD000740 | Pride

Identify the expression of lncRNAs in peripheral blood mononuclear cells from rheumatoid arthritis

Project description:The expression profiles of lncRNAs in peripheral blood mononuclear cells (PBMCs) from rheumatoid arthritis (RA) patients have not been well researched. Therefore, we identified the lncRNA expression profiles in PBMCs from female patients with RA and explored the possible role of the abundantly expressed lncRNAs in RA.

2017-07-12 | GSE101193 | GEO

Sen2020 - Metabolic alteration in PBMCs associated with type 1 diabetes

Project description:Genome-Scale draft model for Human Peripheral Blood Mononuclear Cells (PBMCs). A GEM for PBMCs was developed by applying the INIT algorithm on Human Metabolic Reconstruction (HMR 2.0) as a template model. GEMs were contextualised/ constrained for different conditions using expression datasets. The gene/transcript expression data obtained from PBMCs of Type 1 Diabetes progressors, non-progressors, and healthy controls were employed to score each reaction of HMR 2.0. For further detail please refer to Electronic Supplementary Information of Sen et.al, Metabolic alterations in immune cells associate with progression to type 1 diabetes, Diabetologia, 15/01/2020, (https://doi.org/10.1007/s00125-020-05107-6).

2020-03-11 | MODEL1905270001 | BioModels

Gene expression of human peripheral blood cells of patients with rheumatoid arthritis [dataset 2]

Project description:Factors responsible for radiographic severity of rheumatoid arthritis (RA) in African-Americans are poorly understood. We sought to examine genes whose expression in peripheral blood mononuclear cells (PBMCs) is associated with radiographic severity of RA.

2015-12-31 | GSE64707 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data