Transcriptomics,Genomics

Dataset Information

38

Global gene expression of the rodent malaria parasites Plasmodium yoelii, Plasmodium berghei and Plasmodium chabaudi blood-stage parasites


ABSTRACT: The purpose of this research is to identify and evaluate the global gene expression of the rodent malaria parasites Plasmodium yoelii, Plasmodium berghei and Plasmodium chabaudi blood-stage parasites and specifically compare the blood stage gene expression profiles of samples derived from previous studies on Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi Overall design: Mice were infected by intraperitoneal injections of P. berghei ANKA, P. chabaudi AS or P. yoelii 17x parasitized erythrocytes and parasitaemia and parasite stages were monitored by thin blood smears stained with Giemsa. Mice infected with P. chabaudi were highly synchronized and terminal bled every 2 hours under anesthesia over the course of 24 hr. For P. berghei and P. yoelii infection, mice were terminal bleed and the stage-specific parasitized erythrocytes were separated via Nycodenz density gradient. The ring stage interface was isolated, washed and subjected to ex vivo culture, which was then collected every 2 hr over the course of 24 hr over a complete IDC life-cycle. Samples labeled with Cy5 were hybridized against a reference RNA pool labeled with Cy3, consisting of equal amounts of P. yoelii or P. berghei or P. chabaudi RNA from each time point.

INSTRUMENT(S): ZB/SBS-NTU Plasmodium Rodent YBC 11.6K v1

SUBMITTER: Regina Hoo  

PROVIDER: GSE80015 | GEO | 2016-04-08

SECONDARY ACCESSION(S): PRJNA317634

REPOSITORIES: GEO

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Publications


The genome sequence available for different Plasmodium species is a valuable resource for understanding malaria parasite biology. However, comparative genomics on its own cannot fully explain all the species-specific differences which suggests that other genomic aspects such as regulation of gene expression play an important role in defining species-specific characteristics. Here, we developed a comprehensive approach to measure transcriptional changes of the evolutionary conserved syntenic orth  ...[more]

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