Dataset Information



ABSTRACT: Functional genomics of B. japonicum exposed to osmotic stress caused by 50 mM NaCl. Keywords: stress response Overall design: Three biological replicates were prepared. However, total 4 arrays were hybridized with the dye swap.

INSTRUMENT(S): USDA Bradyrhizobium japonicum 8.5K (BJ_USDA110_Oligo_Array)

SUBMITTER: Woo-Suk Chang  

PROVIDER: GSE8036 | GEO | 2007-07-26



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An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum.

Chang Woo-Suk WS   Franck William L WL   Cytryn Eddie E   Jeong Sooyoung S   Joshi Trupti T   Emerich David W DW   Sadowsky Michael J MJ   Xu Dong D   Stacey Gary G  

Molecular plant-microbe interactions : MPMI 20071001 10

A DNA microarray, comprising 70-mer oligonucleotides, representing 8,453 open reading frames (ORFs), was constructed based on the Bradyrhizobium japonicum strain USDA110 genomic sequence. New annotation predicted 199 additional genes, which were added to the microarray and were shown to be transcribed. These arrays were used to profile transcription in cells under a variety of conditions, including growth in minimal versus rich medium, osmotic stress, and free-living cells versus bacteroids. Inc  ...[more]

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