Genomics

Dataset Information

0

The role of miR-17-92 in the miRegulatory landscape of Ewing Sarcoma (PARCLIP-Seq)


ABSTRACT: MicroRNAs serve to fine-tune gene expression and play an important regulatory role in tissue specific gene networks. The identification and validation of miRNA target genes in a tissue still poses a significant problem since the presence of a seed sequence in the 3´UTR of an mRNA and its expression modulation upon ectopic expression of the miRNA do not reliably predict regulation under physiological conditions. The chimeric oncoprotein EWS-FLI1 is the driving pathogenic force in Ewing Sarcoma. miR-17-92, one of the most potent oncogenic miRNAs, was recently reported to be the top EWS-FLI1 activated miRNA. Using a combination of AGO2 pull-down experiments by PAR-CLIP (Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) and of RNAseq upon miRNA depletion by ectopic sponge expression, we aimed to identify the targetome of miR-17-92 in Ewing sarcoma. Intersecting both datasets we found an enrichment of PAR-CLIP hits for members of the miR-17-92 cluster in the 3´UTRs of genes up-regulated in response to mir-17-92 specific sponge expression. Strikingly, approximately a quarter of these genes annotate to the TGFB/BMP pathway, the majority mapping downstream of SMAD signalling. Taken together, our findings shed light on the complex miRegulatory landscape of Ewing Sarcoma pointing miR-17-92 as a key node connected to TGFB/BMP pathway

ORGANISM(S): Homo sapiens

PROVIDER: GSE80493 | GEO | 2017/01/10

SECONDARY ACCESSION(S): PRJNA319049

REPOSITORIES: GEO

Similar Datasets

2017-01-10 | GSE80492 | GEO
2024-01-26 | PXD041033 | Pride
2022-05-12 | PXD027309 | Pride
| E-GEOD-61944 | biostudies-arrayexpress
| E-GEOD-64686 | biostudies-arrayexpress
| E-GEOD-60890 | biostudies-arrayexpress
| E-GEOD-60889 | biostudies-arrayexpress
2018-09-16 | GSE73092 | GEO
2015-12-31 | GSE64686 | GEO
2018-07-25 | GSE108631 | GEO