Transcriptomics,Genomics

Dataset Information

38

Characterization of primary isolated villous CTB and proximal column EVT from first trimester human placenta.


ABSTRACT: The goal of this study was to identify the gene expression signatures of two closely related types of trophoblast in human placentas: villous cytotrophoblasts (vCTB) and proximal column extravillous trophoblasts (pcEVT). The two populations were isolated from first trimester placentas and identified using the specific surface markers, EGFR (vCTB) and HLA-G (pcEVT). Overall design: First trimester trophoblasts were isolated from 6-9 week gestational age placentas and sorted into EGFR+/HLAG- vCTB and EGFR-/HLAG+ pcEVT. Whole genome expression analysis was performed on purified RNA of the two sorted populations.

INSTRUMENT(S): Illumina HumanHT-12 V4.0 expression beadchip

SUBMITTER: Mana Parast  

PROVIDER: GSE80996 | GEO | 2017-02-01

SECONDARY ACCESSION(S): PRJNA320253

REPOSITORIES: GEO

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Publications

Hypoxia Directs Human Extravillous Trophoblast Differentiation in a Hypoxia-Inducible Factor-Dependent Manner.

Wakeland Anna K AK   Soncin Francesca F   Moretto-Zita Matteo M   Chang Ching-Wen CW   Horii Mariko M   Pizzo Don D   Nelson Katharine K KK   Laurent Louise C LC   Parast Mana M MM  

The American journal of pathology 20170204 4


Villous cytotrophoblasts are epithelial stem cells of the early human placenta, able to differentiate either into syncytiotrophoblasts in floating chorionic villi or extravillous trophoblasts (EVTs) at the anchoring villi. The signaling pathways regulating differentiation into these two lineages are incompletely understood. The bulk of placental growth and development in the first trimester occurs under low oxygen tension. One major mechanism by which oxygen regulates cellular function is throug  ...[more]

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