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Directed differentiation of human embryonic stem cells to corneal endothelial cell-like cells: A transcriptomic analysis

ABSTRACT: The corneal endothelium is composed of a monolayer of corneal endothelial cells (CECs), which is essential for maintaining corneal transparency. Human embryonic stem cells (hESCs) hold the promise of providing an abundant donor source for generating CEC cells for cell replacement therapies. Here we demonstrate that CEC-like cells can be efficiently derived from human ESCs. In addition, we performed global gene expression profiling of stem-cell-derived CEC cells, incorporating with adult CEC cells, fetal CEC cells and other human tissue type data. Our data indicate that hESC-derived CEC-like cells closely resemble human fetal CEC cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE81474 | GEO | 2017/05/07

SECONDARY ACCESSION(S): PRJNA324132

REPOSITORIES: GEO

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