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Directed differentiation of human embryonic stem cells to corneal endothelial cell-like cells: A transcriptomic analysis

ABSTRACT: The corneal endothelium is composed of a monolayer of corneal endothelial cells (CECs), which is essential for maintaining corneal transparency. Human embryonic stem cells (hESCs) hold the promise of providing an abundant donor source for generating CEC cells for cell replacement therapies. Here we demonstrate that CEC-like cells can be efficiently derived from human ESCs. In addition, we performed global gene expression profiling of stem-cell-derived CEC cells, incorporating with adult CEC cells, fetal CEC cells and other human tissue type data. Our data indicate that hESC-derived CEC-like cells closely resemble human fetal CEC cells. Overall design: We analyzed a total of 44 samples. 6 samples were sequenced by our group. High-throughput RNA-seq data from human ES cell-derived CEC-like cells samples (n=4) and cultured adult CEC samples (n=2) using Illumina HiSeq 2000. 38 samples are publicly available in GEO. The entire set of processed data is linked below as supplementary file 44_samples_RPKM.txt.

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)


PROVIDER: GSE81474 | GEO | 2017-05-07



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Directed differentiation of human embryonic stem cells to corneal endothelial cell-like cells: A transcriptomic analysis.

Song Qinglu Q   Yuan Songtao S   An Qin Q   Chen Yinyin Y   Mao Frank Fuxiang FF   Liu Yizhi Y   Liu Qinghuai Q   Fan Guoping G  

Experimental eye research 20160812

Corneal endothelial cells (CECs) are a monolayer of cells covering the inner-side of cornea, playing a pivotal role in keeping the cornea transparent. Because adult CECs have no proliferative capacity, the loss of CECs during aging or under pathological conditions would lead to corneal edema, eventually leading to the blindness. Clinically, donated CECs have been successfully transplanted to treat the diseases of CEC deficiency; however, the source of CEC donation is very limited. As an alternat  ...[more]

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