Dataset Information


ChIP-seq of HSF-1 and Pol II in C. elegans larval development and heat shock

ABSTRACT: To understand the function and regulation of the C. elegans heat shock factor (HSF-1) in larval development, we have used ChIP-seq to analyze the occupancy of HSF1 and RNA Pol II in L2 larvae and young adult (YA) animals grown at 20°C or upon heat shock at 34°C for 30 min. In addition, we have used RNA-seq to analyze the transcriptomes of wild type (N2), hsf-1(ok600) mutants and hsf-1(ok600); rmSi1[hsf-1::gfp] L2 larvae grown at 20°C and characterized the gene expression change by heat shock in wild type (N2) animals at L2 stage. Overall design: Experiment type: ChIP-seq. Biological Source: strain: AM1061; developmental dtage: L2 Larva, young adult (YA). Experimental Factors: temperature: 20 degree celsius, 34 degree celsius; antibodies: anti-GFP (clontech, #632592), anti-AMA-1 (Novus, #38520002).

INSTRUMENT(S): Illumina HiSeq 2000 (Caenorhabditis elegans)


PROVIDER: GSE81521 | GEO | 2016-10-12



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E2F coregulates an essential HSF developmental program that is distinct from the heat-shock response.

Li Jian J   Chauve Laetitia L   Phelps Grace G   Brielmann Renée M RM   Morimoto Richard I RI  

Genes & development 20160929 18

Heat-shock factor (HSF) is the master transcriptional regulator of the heat-shock response (HSR) and is essential for stress resilience. HSF is also required for metazoan development; however, its function and regulation in this process are poorly understood. Here, we characterize the genomic distribution and transcriptional activity of Caenorhabditis elegans HSF-1 during larval development and show that the developmental HSF-1 transcriptional program is distinct from the HSR. HSF-1 developmenta  ...[more]

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