Dataset Information


Transcriptional profiling of NMB0419 mutant of Neisseria meningitidis MC58

ABSTRACT: Neisseria meningitidis is an obligate commensal colonising the human nasopharynx and occasionally invades the bloodstream causing life-threatening meningitis and septicaemia. The gene NMB0419 on the genome of N. meningitidis MC58 encodes a putative Sel1-like repeat (SLR) containing protein, which has been implicated in mediating meningococcal invasion of epithelial cells. We prepared RNA samples from N. meningitidis MC58 (WT) and its isogenic mutant of NMB0419 grown to log phase in in-vitro culture. The RNA samples were subjected to RNA sequencing. The resulting transcriptomes were compared to determine the genes that differentially expressed in NMB0419 mutant. Overall design: RNA samples werer prepared from three biological replicates for NMB0419 mutant strain and wild type (WT) MC58 strain and were subjected to paired comparison between NMB0419 mutant and WT strain.

INSTRUMENT(S): Illumina HiSeq 2000 (Neisseria meningitidis MC58)

SUBMITTER: Ming-Shi Li  

PROVIDER: GSE81572 | GEO | 2017-03-07



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Inactivation of NMB0419, Encoding a Sel1-Like Repeat (SLR) Protein, in Neisseria meningitidis Is Associated with Differential Expression of Genes Belonging to the Fur Regulon and Reduced Intraepithelial Replication.

Li Ming-Shi MS   Langford Paul R PR   Kroll J Simon JS  

Infection and immunity 20170421 5

Neisseria meningitidis is a commensal microbe that colonizes the human nasopharynx but occasionally invades the bloodstream to cause life-threatening infection. N. meningitidis MC58 NMB0419 encodes a Sel1-like repeat (SLR)-containing protein, previously implicated in invasion of epithelial cells. A gene-regulatory function was revealed in Escherichia coli expressing plasmid-borne NMB0419 and showing significantly increased epithelial adherence compared to the wild type, due to increased expressi  ...[more]

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