Dataset Information


Rhodobacter sphaeroides 2.4.1 pPos19 versus pBBR under singlet oxygen stress

ABSTRACT: To learn more about the function of Pos19, we constructed an over-expression strain. For this purpose, the Pos19 gene together with its native RpoE promoter was cloned into the middle-copy plasmid pBBR1 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a singlet oxygen-induced Pos19 over-expression compared to an empty vector control. Total RNA from the over-expression (pPos19) and from the empty vector control (pBBR) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate Pos19-induced changes on transcriptome level. Overall design: RNA samples collected after 7 min of singlet oxygen stress were analyzed by two-color microarrays. Each microarray contains a pool of three independent biological replicates for each sample.

INSTRUMENT(S): Agilent-27061/Klug lab - Rhodobacter sphaeroides - 15k array Version 2 (144 sRNA) (condensed Probe Identifier version)

SUBMITTER: Gabriele Klug  

PROVIDER: GSE81718 | GEO | 2017-02-09



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Characteristics of Pos19 - A Small Coding RNA in the Oxidative Stress Response of Rhodobacter sphaeroides.

Müller Katrin M H KM   Berghoff Bork A BA   Eisenhardt Benjamin D BD   Remes Bernhard B   Klug Gabriele G  

PloS one 20160926 9

The phototrophic bacterium Rhodobacter sphaeroides induces several small RNAs (sRNAs) when singlet oxygen (1O2) levels are elevated, a situation also referred to as photo-oxidative stress. An RNA-seq study identified the RSs0019 sRNA, which is renamed Pos19 (photo-oxidative stress induced sRNA 19). Pos19 is part of the RpoE regulon and consequently induced upon 1O2 and peroxide stress. The 219 nt long Pos19 transcript contains a small open reading frame (sORF) of 150 nt, which is translated in v  ...[more]

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