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18

RpoE regulon in Rhodobacter sphaeroides


ABSTRACT: In Rhodobacter sphaeroides a transcriptional response to the reactive oxygen species singlet oxygen is controlled by the group IV sigma factor RpoE and the anti-sigma factor ChrR. In this study, we integrated various large datasets to identify genes within the singlet oxygen stress response that contain RpoE-dependent promoters within R. sphaeroides. Transcript pattern clustering and a RpoE-binding sequence model were used to predict candidate promoters that respond to singlet oxygen stress in R. sphaeroides. These candidate promoters were experimentally validated to nine R. sphaeroides RpoE-dependent promoters that control the transcription of 15 genes activated by singlet oxygen. Overall design: DNA immunoprecipitated with polyclonal antibodies against RpoE or the Beta' subunit of RNA polymerase was labelled with Cy5 and hybridized on two-color tilling arrays (triplicates for each) with genomic DNA as an input control labelled with Cy3.

INSTRUMENT(S): UW_Rhodobacter_sphaeroides_2.4.1_385K_v2

ORGANISM(S): Rhodobacter sphaeroides 2.4.1  

SUBMITTER: Yann S Dufour  

PROVIDER: GSE22576 | GEO | 2010-07-01

SECONDARY ACCESSION(S): PRJNA128415

REPOSITORIES: GEO

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