RpoE regulon in Rhodobacter sphaeroides

ABSTRACT: In Rhodobacter sphaeroides a transcriptional response to the reactive oxygen species singlet oxygen is controlled by the group IV sigma factor RpoE and the anti-sigma factor ChrR. In this study, we integrated various large datasets to identify genes within the singlet oxygen stress response that contain RpoE-dependent promoters within R. sphaeroides. Transcript pattern clustering and a RpoE-binding sequence model were used to predict candidate promoters that respond to singlet oxygen stress in R. sphaeroides. These candidate promoters were experimentally validated to nine R. sphaeroides RpoE-dependent promoters that control the transcription of 15 genes activated by singlet oxygen. DNA immunoprecipitated with polyclonal antibodies against RpoE or the Beta' subunit of RNA polymerase was lab

ORGANISM(S): Rhodobacter sphaeroides 2.4.1

SUBMITTER: Yann Dufour 

PROVIDER: E-GEOD-22576 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress