Post-replicative formation of cohesion is required for repair and induced by a single DNA break

ABSTRACT: Sister chromatid cohesion, established during replication by the protein complex Cohesin, is essential for both chromosome segregation and double-strand break (DSB) repair. Normally cohesion formation is strictly limited to the S-phase of the cell cycle, but DSBs can trigger cohesion also after DNA replication has been completed. The function of this damage-induced cohesion remains unknown. In this investigation we show that it is essential for repair in post-replicative cells in yeast. Furthermore, it is established genome-wide after induction of a single DSB, and controlled by the DNA damage response and Cohesin regulating factors. We thus define a cohesion establishment pathway that is independent of DNA duplication and acts together with cohesion formed during replication in sister chromatid-based DSB repair. Keywords: ChIP-chip analysis

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE8270 | GEO | 2007/07/13

SECONDARY ACCESSION(S): PRJNA101223

REPOSITORIES: GEO