Transcriptomics

Dataset Information

26

Characterization of weight loss and weight regain mechanisms after Roux-en-Y gastric bypass in rats


ABSTRACT: Roux-en-Y gastric bypass (RYGB) is the most effective therapy for morbid obesity, but it has a ~20% failure rate. We used our established RYGB model in diet-induced obese (DIO) Sprague-Dawley rats, which reproduces human bi-phasic body weight (BW) loss pattern, to determine mechanisms contributing to success (RGYB-S) or failed (RYGB-F) RYGB. DIO rats were randomized to RYGB, sham operated Obese, and sham operated obese pair fed-linked to RYGB (PF) groups. BW, caloric intake (CI) and fecal output (FO) were recorded daily for 90 days, food efficiency (FE) was calculated, and morphologic changes were determined. Gut, adipose and thyroid hormones were measured in plasma. Mitochondrial respiratory complexes in skeletal muscle, expression of energy-related hypothalamic and fat peptides, receptors and enzymes were quantified. A 25% failure rate occurred. RYGB-S, RYGB-F and PF rats vs. Obese showed rapid BW decrease, followed by sustained BW loss in RYGB-S. RYGB-F and PF gradually increased BW. Expression profiling of both CNS (hypothalamus) and peripheral tissues (subcutaneous abdominal fat) strongly supported the involvement of a number of metabolic and feeding-related genes in the differential outcomes. Keywords: gene expression analysis Overall design: 3 biological replicate RNA samples were prepared from 2 tissues (the subcutaneous abdominal fat or the hypothalamus) from rats in 3 treatment groups (rats losing weight successfully after gastric bypass surgery, rats gaining weight, and rats that were fed the same amount as the treated rats)

INSTRUMENT(S): [Rat230_2] Affymetrix Rat Genome 230 2.0 Array

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Frank A Middleton  

PROVIDER: GSE8314 | GEO | 2007-06-30

SECONDARY ACCESSION(S): PRJNA101291

REPOSITORIES: GEO

Similar Datasets

2008-06-16 | E-GEOD-8314 | ArrayExpress
2015-05-13 | E-GEOD-68812 | ArrayExpress
2007-08-07 | GSE8700 | GEO
2007-10-26 | E-GEOD-5109 | ArrayExpress
2014-09-03 | E-GEOD-61005 | ArrayExpress
2017-12-13 | PXD008071 | Pride
2015-07-29 | E-GEOD-5109 | ExpressionAtlas
2009-04-23 | E-GEOD-8700 | ArrayExpress
| GSE76944 | GEO
| GSE76468 | GEO