Project description:Expression analysis in HepG2 and IMR90 cells in presence of Fluc siRNA or sPom121 or Nup98 siRNA Transfect siRNA - extract RNA 72 hrs post transfection
Project description:DamID with sPom121, Nup98 and control proteins (GFP and Cbx1) N-term tag sPom121, Nup98 or GFP and express in HeLa-C cells - conduct deep sequencing
Project description:One-third of all human cancers are attributable to mutations in RAS. Induction of oncogenic RAS during tumorigenesis leads to metabolic reprogramming and enhanced proliferation or senescence. These profound phenotypic changes require adaptations in gene expression, RNA processing, and protein synthesis. To investigate core drivers of this transformation, we analysed nuclear protein expression in IMR90 RAS cells and IMR90 Control cells 6 days after induction of HRASG12V.
Project description:We profiled transcriptomes of IR‑induced senescent IMR90 fibroblasts subjected to siRNA‑mediated knockdown of CCND1, CDK4, CDK6 (and CDK4+6), alongside two non‑targeting controls. Transfections occurred on days 4/5 and 7/8 post‑IR; all samples were harvested day 10 for RNA extraction. Differential expression identifies kinase‑specific roles in sustaining inflammatory and DNA damage responses in senescence.
Project description:The objective was to evaluate the status of the global hepatic transcriptome in the presence of inhibited calreticulin levels. Results identify a total of 324 genes were altered of which 245 genes were downregulated and 79 genes were upregulated. This suggests that such altered genes might be associated with altered hepatic physiology that are observed during calreticulin deficiency. Total RNA was isolated from HepG2 cells subjected to calreticulin (CRT) siRNA for 24 hours compared to control HepG2 cells subjected to scramble siRNA for 24 hours. This was then hybridized to Illumina HumanHT-12 v4 Expression BeadChip arrays.
Project description:The goal of this study was to analyse changes of splicing and gene expression induced in IMR90 Control and IMR90 RAS (HRASG12V) cells upon knockdown of splicing factor SF3B1.
Project description:To gain insight into possible processes that require m6A for their function, METTL3 was knocked down (KD) in HepG2 cells by siRNA transfections Differential expression analysis of METTL3 KD versus mock-transfected HepG2 cells, in 2 biological replicates