Transcriptomics,Genomics

Dataset Information

44

Genome-wide transcript analysis of Bradyrhizobium japonicum bacteroids in soybean root nodules


ABSTRACT: Expression data from B. japonicum soybean root nodules including a nodulation time-course experiment with soybean nodules harvested at 10, 13, 21 and 31 dpi and transcriptome of bacteroids formed by a mutant defective in the RNA polymerase transcription factor sigma 54. Two reference data sets were established using B. japonicum cells grown in PSY medium under either aerobic or micro-aerobic conditions. Keywords: genetic modification, time course, growth conditions Overall design: B. japonicum transcriptome was determined under free-living and symbiotic conditions including mutant analysis (sigma 54) and nodulation time-course experiments

INSTRUMENT(S): Affymetrix custom Bradyrhizobium japonicum strain USDA 110 19K array

SUBMITTER: Hubert Rehrauer  

PROVIDER: GSE8478 | GEO | 2007-07-14

SECONDARY ACCESSION(S): PRJNA105365

REPOSITORIES: GEO

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Publications

Genome-wide transcript analysis of Bradyrhizobium japonicum bacteroids in soybean root nodules.

Pessi Gabriella G   Ahrens Christian H CH   Rehrauer Hubert H   Lindemann Andrea A   Hauser Felix F   Fischer Hans-Martin HM   Hennecke Hauke H  

Molecular plant-microbe interactions : MPMI 20071101 11


The transcriptome of endosymbiotic Bradyrhizobium japonicum bacteroids was assessed, using RNA extracted from determinate soybean root nodules. Results were compared with the transcript profiles of B. japonicum cells grown in either aerobic or microaerobic culture. Microoxia is a known trigger for the induction of symbiotically relevant genes. In fact, one third of the genes induced in bacteroids at day 21 after inoculation are congruent with those up-regulated in culture by a decreased oxygen c  ...[more]

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