Genomics

Dataset Information

158

Massively multiplex single-cell Hi-C


ABSTRACT: Proof-of-concept for a massively parallel single-cell version of the Hi-C protocol Overall design: Single-cell Hi-C Raw data for GSM2254215, GSM2254216, GSM2254218, GSM2254219 and GSM2438426 were submitted to dbGaP due to patient privacy concerns. The raw data are available at dbGaP accession ID phs001269.v1.p1.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens; Mus musculus)

SUBMITTER: Vijay Ramani 

PROVIDER: GSE84920 | GEO | 2017-01-28

SECONDARY ACCESSION(S): PRJNA335638

REPOSITORIES: GEO

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Publications


We present single-cell combinatorial indexed Hi-C (sciHi-C), a method that applies combinatorial cellular indexing to chromosome conformation capture. In this proof of concept, we generate and sequence six sciHi-C libraries comprising a total of 10,696 single cells. We use sciHi-C data to separate cells by karyotypic and cell-cycle state differences and identify cell-to-cell heterogeneity in mammalian chromosomal conformation. Our results demonstrate that combinatorial indexing is a generalizabl  ...[more]

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