Transcriptomics,Genomics

Dataset Information

17

Expression differences between worker(female) and drone(male) honey bee antennae


ABSTRACT: We have identified a honeybee (Apis mellifera) odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA) from four candidate sex pheromone odorant receptors from the honeybee genome based on their biased expression in drone antennae. Keywords: Tissue Comparison Overall design: Oligonucleotides (70-mer sense-strand) were designed using ArrayOligoSelector3.5 software (43, http://sourceforge.net) using default settings and the Apis mellifera genome (Assemby 2) for the following conceptual cDNA categories: 168 odorant receptors; 49 cytochrome p450s; 19 odorant binding proteins; 17 tetraspanins; 10 gustatory receptors; 10 carboxyl esterases; 7 glutathione S transferases; 6 chemosensory proteins (sensory appendage proteins); 5 lipocalins; 5 visual and non-visual opsins; 4 alpha-arrestins; 4 arrestins; 3 G-protein alpha subunits; 1 Pinocchio ortholog (a Drosophila melanogaster sensory-related protein); 1 sensory neuron membrane protein, and 4 housekeeping genes (cytoplasmic actin, EF1alpha, ribosomal protein 49, ribosomal protein S3), for a total of 313 genes. ArrayControl sense 70-mer oligonucleotides (Ambion) were printed as negative control spots. Four microarray hybridizations (replicates) were performed using mature drone antennal total RNA versus worker antennal total RNA with an even dye swap. Each total RNA pool was derived from an independent collection of 50 sets of antennae.

INSTRUMENT(S): UIUC/WMKCCFG Honeybee Sensory/CYP450 0.7K v1.0

SUBMITTER: Hugh Robertson  

PROVIDER: GSE8519 | GEO | 2007-08-09

SECONDARY ACCESSION(S): PRJNA101639

REPOSITORIES: GEO

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Publications

A honey bee odorant receptor for the queen substance 9-oxo-2-decenoic acid.

Wanner Kevin W KW   Nichols Andrew S AS   Walden Kimberly K O KK   Brockmann Axel A   Luetje Charles W CW   Robertson Hugh M HM  

Proceedings of the National Academy of Sciences of the United States of America 20070830 36


By using a functional genomics approach, we have identified a honey bee [Apis mellifera (Am)] odorant receptor (Or) for the queen substance 9-oxo-2-decenoic acid (9-ODA). Honey bees live in large eusocial colonies in which a single queen is responsible for reproduction, several thousand sterile female worker bees complete a myriad of tasks to maintain the colony, and several hundred male drones exist only to mate. The "queen substance" [also termed the queen retinue pheromone (QRP)] is an eight-  ...[more]

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