Genomics

Dataset Information

43

Gene expression profiles from human splenic Marginal Zone and Naive (Follicular) B cells


ABSTRACT: Splenic Marginal Zone B cells mount innate-like T cell-independent (TI) antibody responses to blood-borne antigens upon receiving activation signals from TACI-engaging factors BAFF and APRIL. The transcriptome analysis of splenic Marginal Zone (MZ) B cells was performed to verify which transcriptional programs are enhanced in the innate-like MZ B cell subset and to study the involvement of mTOR in MZ B cells activated via TACI. Overall design: Unstimulated human Marginal Zone and Follicular/Naive B cells were FACSorted from spleens of seven (MZ) and three (FO) adult healthy subjects for RNA isolation and Agilent analysis. Moreover, APRIL (500ng/ml) and Rapamycin (10nM) were used to dissect the involvement of mTOR in the induction of Class Switch Recombination and antibody production in MZ B cells activated through TACI.

INSTRUMENT(S): Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version)

SUBMITTER: Jordi Sintes  

PROVIDER: GSE85289 | GEO | 2017-10-14

SECONDARY ACCESSION(S): PRJNA338083

REPOSITORIES: GEO

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Publications


Mechanistic target of rapamycin (mTOR) enhances immunity in addition to orchestrating metabolism. Here we show that mTOR coordinates immunometabolic reconfiguration of marginal zone (MZ) B cells, a pre-activated lymphocyte subset that mounts antibody responses to T-cell-independent antigens through a Toll-like receptor (TLR)-amplified pathway involving transmembrane activator and CAML interactor (TACI). This receptor interacts with mTOR via the TLR adapter MyD88. The resulting mTOR activation in  ...[more]