Genomics

Dataset Information

0

Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant


ABSTRACT: The 7B-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. Transcriptomic profiles of the 7B-1 male-sterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 up-regulated and 636 down-regulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 7B-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 7B-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 7B-1 anthers. The present study provides insights into the transcriptome of the 7B-1 mutant. We identified several genes with altered expression level in 7B-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.

ORGANISM(S): Solanum lycopersicum

PROVIDER: GSE85859 | GEO | 2017/02/09

SECONDARY ACCESSION(S): PRJNA339564

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-43982 | biostudies-arrayexpress
| E-GEOD-51779 | biostudies-arrayexpress
2013-05-01 | GSE43982 | GEO
2008-09-16 | GSE12756 | GEO
| E-GEOD-12756 | biostudies-arrayexpress
| E-GEOD-29217 | biostudies-arrayexpress
| E-MTAB-4277 | biostudies-arrayexpress
2021-01-13 | PXD002756 | Pride
| E-GEOD-53016 | biostudies-arrayexpress
| E-GEOD-23615 | biostudies-arrayexpress