Transcriptomics,Genomics

Dataset Information

144

RNA-seq transcriptonal profiling in E13.5 fetal liver erythroid cells from Tfam WT and KO mice


ABSTRACT: The developing erythroid cells require highly coordinated gene expression and metabolism. By comparing the proteomic and transcriptomic changes in human hematopoietic stem/progenitor cells (HSPCs) and lineage-committed erythroid progenitors (ProEs), and uncover pathways related to mitochondrial biogenesis enhanced through post-transcriptional regulation. Two principal mitochondrial factors TFAM and PHB2 are tightly regulated at the protein level and indispensable for mitochondria and erythropoiesis. To determine the role of TFAM in mitochondrial function during erythroid development, we generated Tfam conditional knockout (KO) mice by an erythroid-specific EpoR-Cre allele. We isolated the CD71+Ter119+ embryonic day (E)13.5 fetal liver erythroid cells by FACS sorting, and performed RNA-seq transcriptional profiling analysis. Overall design: CD71+Ter119+ embryonic day (E)13.5 fetal liver erythroid cells were isolated by FACS sorting. Total RNA were extracted and processed for RNA-seq transcriptional profiling analysis.

INSTRUMENT(S): Illumina NextSeq 500 (Mus musculus)

SUBMITTER: Jian Xu  

PROVIDER: GSE86865 | GEO | 2017-05-15

SECONDARY ACCESSION(S): PRJNA342800

REPOSITORIES: GEO

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Publications


Advances in genomic profiling present new challenges of explaining how changes in DNA and RNA are translated into proteins linking genotype to phenotype. Here we compare the genome-scale proteomic and transcriptomic changes in human primary haematopoietic stem/progenitor cells and erythroid progenitors, and uncover pathways related to mitochondrial biogenesis enhanced through post-transcriptional regulation. Mitochondrial factors including TFAM and PHB2 are selectively regulated through protein  ...[more]

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