Genomics

Dataset Information

156

Biogeographic conservation and CO2-dynamics of the cytosine methylome in the globally important cyanobacterium Trichodesmium [WGBS]


ABSTRACT: Our paper presents the results of a study in which we used whole genome bisulfite sequencing (WGBS), RNA-Seq (i.e. transcriptomics), high-CO2 physiology experiments, and spatiotemporally separated samples isolated in situ (i.e. directly from the ocean) to examine the metabolic potential of genome-wide cytosine (5mC) methylation (i.e. epigenomics), its potential impacts to transcriptional dynamics under both present-day and future ocean acidification conditions, and its biogeographic conservation in the globally-significant, biogeochemically-critical marine cyanobacterium Trichodesmium. Overall design: Characterize the 5mC cytosine methylation landscape of Trichodesmium erythraeum IMS101 and examine its relationship to its transcriptome under both 380 and 750 µatm CO2, respectively. WGBS of other T. erythraeum isolates (IMSB and 2175) as well as samples from natural populations isolated in situ in order to biogeographically characterize the Trichodesmium cytosine methylome.

INSTRUMENT(S): Illumina NextSeq 500 (Trichodesmium erythraeum IMS101)

SUBMITTER: Eric Alva Webb  

PROVIDER: GSE86979 | GEO | 2018-03-01

REPOSITORIES: GEO

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Publications

Biogeographic conservation of the cytosine epigenome in the globally important marine, nitrogen-fixing cyanobacterium Trichodesmium.

Walworth Nathan G NG   Hutchins David A DA   Dolzhenko Egor E   Lee Michael D MD   Fu Feixue F   Smith Andrew D AD   Webb Eric A EA  

Environmental microbiology 20171102 11


Cytosine methylation has been shown to regulate essential cellular processes and impact biological adaptation. Despite its evolutionary importance, only a handful of bacterial, genome-wide cytosine studies have been conducted, with none for marine bacteria. Here, we examine the genome-wide, C5 -Methyl-cytosine (m5C) methylome and its correlation to global transcription in the marine nitrogen-fixing cyanobacterium Trichodesmium. We characterize genome-wide methylation and highlight conserved moti  ...[more]

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