Transcriptomics,Genomics

Dataset Information

41

Conversion of terminally committed mouse hepatocytes to culturable bipotent progenitor cells


ABSTRACT: A challenge for advancing approaches to liver regeneration is loss of functional differentiation capacity when hepatocyte progenitors are maintained in culture. Recent lineage-tracing studies have shown that mature hepatocytes (MHs) convert to an immature state during chronic liver injury, and we investigated whether this conversion could be recapitulated in vitro and if such converted cells could represent a source of expandable hepatocytes. We report that a cocktail of small molecules, Y-27632, A-83-01 and CHIR99021, can convert rat and mouse MHs in vitro into proliferative bipotent cells, which we term chemically induced liver progenitors (CLiPs). CLiPs can differentiate into both MHs and biliary epithelial cells that can form functional ductal structures. CLiPs in long-term culture did not lose their proliferative capacity or their hepatic differentiation ability, and rat CLiPs were shown to extensively repopulate chronically injured liver tissue. Thus our study advances the goals of liver regenerative medicine. Transcriptomic analysis for mouse CLiPs at P1 which underwent hepatic induction (Matrix 1). Transcriptomic analysis for mouse CLiPs at P11-12 which underwent hepatic induction (Matrix 2). Overall design: Hepatic induction was performed for P1 mouse CLiPs or CLiPs that underwent 11-12 passages.

INSTRUMENT(S): Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version]

SUBMITTER: Takeshi Katsuda  

PROVIDER: GSE87579 | GEO | 2016-11-15

SECONDARY ACCESSION(S): PRJNA345376

REPOSITORIES: GEO

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Publications

Conversion of Terminally Committed Hepatocytes to Culturable Bipotent Progenitor Cells with Regenerative Capacity.

Katsuda Takeshi T   Kawamata Masaki M   Hagiwara Keitaro K   Takahashi Ryou-U RU   Yamamoto Yusuke Y   Camargo Fernando D FD   Ochiya Takahiro T  

Cell stem cell 20161110 1


A challenge for advancing approaches to liver regeneration is loss of functional differentiation capacity when hepatocyte progenitors are maintained in culture. Recent lineage-tracing studies have shown that mature hepatocytes (MHs) convert to an immature state during chronic liver injury, and we investigated whether this conversion could be recapitulated in vitro and whether such converted cells could represent a source of expandable hepatocytes. We report that a cocktail of small molecules, Y-  ...[more]

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