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Phosphoproteome and proteome of mouse hepatocyte maturation and human hepatic reprogramming

ABSTRACT: To identify central protein kinases that potentially promote the maturation, we chemically induced liver progenitor cells (CLiPs) from mouse hepatocytes using a previously established protocol, and profiled the phosphoproteome and proteome of freshly isolated primary mouse hepatocytes (MHs) and ALBUMIN+ hepatocytes (CLiP-Hep) cells. To systematically interrogate the early regulatory events of hepatic reprogramming, we profiled the Phosphoproteome and proteome in human dermal fibroblasts (HDFs) at 2.25 days (FHH-2.25d) and 5 days (FHH-5d) after infection of lenti-virus encoding three liver-specific transcription factors, FOXA3, HNF1A and HNF4A (FHH). In this analysis, HDF infected with lenti-virus expressing GFP for 2.25 days (GFP) were used as control.

ORGANISM(S): Homo Sapiens Mus Musculus

SUBMITTER: Yu Xue

PROVIDER: PXD035829 | iProX | Fri Aug 05 00:00:00 BST 2022

REPOSITORIES: iProX

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PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest.

Yuan Yangyang Y Wang Chenwei C Zhuang Xuran X Lin Shaofeng S Luo Miaomiao M Deng Wankun W Zhou Jiaqi J Liu Lihui L Mao Lina L Peng Wenbo W Chen Jian J Wang Qiangsong Q Shu Yilai Y Xue Yu Y Huang Pengyu P

Nature communications 20220906 1

Protein kinase-mediated phosphorylation plays a critical role in many biological processes. However, the identification of key regulatory kinases is still a great challenge. Here, we develop a trans-omics-based method, central kinase inference, to predict potentially key kinases by integrating quantitative transcriptomic and phosphoproteomic data. Using known kinases associated with anti-cancer drug resistance, the accuracy of our method denoted by the area under the curve is 5.2% to 29.5% highe ...[more]

PMID: 36068222

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