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Efficient generation of pancreatic β-like cells from the mouse gallbladder

ABSTRACT: We report here an improved protocol to reprogram mouse gallbladder cells (GBCs) into pancreatic beta cells. To fully understand the extent of reprogramming, mRNA was extracted from FACS-purified MIP-GFP positive insulin-producing cells (namely rGBC2) for RNA-seq after 10-days of in vitro reprogramming. The global gene expression profile of rGBC2 was compared with that of primary gallbladder cells, GBC reprogrammed with the rGBC1 protocol (Hickey et al., 2013) and mouse pancreatic β cells (Benner et al., 2014). We show that rGBC2 from four independent cell batches showed a unique gene expression phenotype. Compared with the rGBC1 protocol, rGBC2 expressed many additional pancreatic β cell genes, suppressed many gallbladder genes and resulted in an expression profile closer to pancreatic β cells. Overall design: Gene expression profiling of galbladder reprogrammed, insulin positive cells using the improved reprogramming protocol

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

ORGANISM(S): Mus Musculus

SUBMITTER: Yuhan Wang  

PROVIDER: GSE87606 | GEO | 2017-01-01



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Efficient generation of pancreatic β-like cells from the mouse gallbladder.

Wang Yuhan Y   Galivo Feorillo F   Pelz Carl C   Haft Annelise A   Lee Jonghyeob J   Kim Seung K SK   Grompe Markus M  

Stem cell research 20161027 3

Direct reprogramming is a promising approach for the replacement of β cells in diabetes. Reprogramming of cells originating from the endodermal lineage, such as acinar cells in the pancreas, liver cells and gallbladder cells has been of particular interest because of their developmental proximity to β cells. Our previous work showed that mouse gallbladder epithelium can be partially reprogrammed in vitro to generate islet-like cells (rGBC1). Here, the reprogramming protocol was substantially imp  ...[more]

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