Project description:Whole transcriptome analysis of dechorinated zebrafish embryos after (static?) exposure to one of five smoke dyes from 6 hours post-fertilization (hpf) to 48 hpf. Smoke dyes tested are Disperse Blue 14, Disperse Red 9, Solvent Red 169, Solvent Violet 47, and Solvent Yellow 33. Results provide insight into the molecular mechanisms of immunological response to the dyes.
Project description:Investigation of microRNA expression profile of 12, 24, 36 and 48 hours post-fertilization Danio rerio embryos developmentally exposed to retinoic acid. A twelve chip study using total RNA recovered from pools of 75 tropical 5D zebrafish embryos at 12, 24, 36, and 48 hours post fertilization (hpf). Embryos were exposed to control embryo medium or 5 nM retinoic acid from 6-24 hpf, with two biological replicates per condition. Control samples were pooled and hybridized to a single array. 12 miRZebrafish arrays (based on MirBase release 12.0) were used to measure the expression level of 218 mature miRNA from Danio rerio.
Project description:The purpose of this study was to identify transcripts differentially expressed in zebrafish embryos exposed to two oxygenated PAHs, 1,9-benz-10-anthrone and benzanthracene-7,12-dione, which cause abnormal development. We used RNA-seq (Illumina HiSeq) to identify mRNA profiles of whole zebrafish embryos exposed to 10 μM 1,9-benz-10-anthrone, benzanthracene-7,12-dione or vehicle control (1% DMSO) from 6-48 hours post fertilization
Project description:To test wether endocrine disruption is detectable in zebrafish at the transcriptome level, we exposed newly fertilized zebrafish embryos to low doses of genistein (EC10 and EC20) for 48 hours.
Project description:Transcriptomic profiling of the response to dioxin in developing zebrafish embryos, at 24, 48, 72, 96, and 120 h post-fertilization. The goal was to elucidate mechanisms by which dioxin causes toxicity in zebrafish; this dose was previously shown to induce teratogenesis.
Project description:During early neurogenesis, multiple whole animal gene expression profiling studies revealed widespread changes in developmental mRNA and miRNA abundance in ethanol-exposed embryos. Consistent with a role for miRNAs in neurobehavioral development, miRNA target prediction analyses identified multiple miRNAs misexpressed in the ethanol exposed cohorts that were also predicted to target inversely expressed transcripts known to influence brain morphogenesis. [mRNA] A twelve chip study using total RNA recovered from pools of 75 tropical 5D zebrafish embryos at 24 hours post fertilization (hpf). Embryos were exposed to control embryo medium, 100 mM ethanol, or 300 mM ethanol from 4-24 hpf, with four-fold biological redundancy per condition. A single 12x135K Nimblegen array was used to measure the expression level of 38,489 genes from Danio rerio using 60-mer probes, with three-fold technical redundancy. [miRNA] A twelve chip study using miRNA recovered from pools of 75 tropical 5D zebrafish embryos at 12, 24, 36, and 48 hours post fertilization (hpf). Embryos were exposed to control embryo medium or 300 mM ethanol from 4-24 hpf, with two-fold biological redundancy per condition. Control samples were pooled and hybridized to a single array. 12 miRZebrafish arrays (based on MirBase release 12.0) were used to measure the expression level of 218 mature miRNA from Danio rerio, with 12-fold technical redundancy.
Project description:Gene respons in the central nervous system of zebrafish embryos exposed to the neurotoxicant methyl mercury Two-condition experiment, control vs MeHg exposure embryos (60 µg/l), Biological replicates: 4, dye swaps