MRNA and miRNA expression analysis of developmental ethanol exposure in zebrafish

ABSTRACT: During early neurogenesis, multiple whole animal gene expression profiling studies revealed widespread changes in developmental mRNA and miRNA abundance in ethanol-exposed embryos. Consistent with a role for miRNAs in neurobehavioral development, miRNA target prediction analyses identified multiple miRNAs misexpressed in the ethanol exposed cohorts that were also predicted to target inversely expressed transcripts known to influence brain morphogenesis. [mRNA] A twelve chip study using total RNA recovered from pools of 75 tropical 5D zebrafish embryos at 24 hours post fertilization (hpf). Embryos were exposed to control embryo medium, 100 mM ethanol, or 300 mM ethanol from 4-24 hpf, with four-fold biological redundancy per condition. A single 12x135K Nimblegen array was used to measure th

ORGANISM(S): Danio rerio

SUBMITTER: Robert Tanguay 

PROVIDER: E-GEOD-30497 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress