Genomics

Dataset Information

44

ArrayCGH from human mesenchymal stem cells (hMSC) and hMSC after adipogenic and osteogenic differentiation


ABSTRACT: hMSCs (Lonza) were differentiated towards adipocytes with three cycles of adipocyte differentiation induction and maintenance. hMSCs were differentiated towards osteocytes with osteogenic differentiation medium for 3 days and for 7 days. ArrayCGH experiments were performed before differentiation induction, after 2 and 3 cycles of adipogenic differentiation induction and after 3 days and 7 days of osteogenic differentiation induction. Overall design: Genomic DNA was isolated form hMSCs before differentiation induction, after 2 and 3 cycles adipogenic differentiation induced cells and from 3-day and 7-day osteogenic differentiation induced cells.

INSTRUMENT(S): Agilent-021529 SurePrint G3 Human CGH Microarray Kit, 1x1M, design ID 21529 [hg19:GRCh37:Feb2009]

SUBMITTER: Valentina Galata 

PROVIDER: GSE88947 | GEO | 2016-10-20

SECONDARY ACCESSION(S): PRJNA349168

REPOSITORIES: GEO

altmetric image

Publications

Gene amplification in mesenchymal stem cells and during differentiation towards adipocytes or osteoblasts.

Altmayer Nora Corinna NC   Galata Valentina V   Warschburger Nadine N   Keller Andreas A   Meese Eckart E   Fischer Ulrike U  

Oncotarget 20171201 2


Gene amplifications are an attribute of tumor cells and have for long time been overlooked in normal cells. A growing number of investigations describe gene amplifications in normal mammalian cells during development and differentiation. Possibly, tumor cells have rescued the gene amplification mechanism as a physiological attribute of stem cells. Here, we investigated human mesenchymal stem cells (hMSCs) for gene amplification using array-CGH, single cell fluorescence in situ hybridization and  ...[more]

Similar Datasets

2014-01-01 | S-EPMC4234627 | BioStudies
2013-01-01 | S-EPMC3765027 | BioStudies
1000-01-01 | S-EPMC3418434 | BioStudies
2020-01-01 | S-EPMC7312587 | BioStudies
2018-01-01 | S-EPMC5832166 | BioStudies
2020-01-01 | S-EPMC7268774 | BioStudies
2008-01-01 | S-EPMC2151969 | BioStudies
2016-01-01 | S-EPMC5000485 | BioStudies
1000-01-01 | S-EPMC2587615 | BioStudies
1000-01-01 | S-EPMC5742872 | BioStudies