Dataset Information


ArrayCGH from human mesenchymal stem cells (hMSC) and hMSC after adipogenic and osteogenic differentiation

ABSTRACT: hMSCs (Lonza) were differentiated towards adipocytes with three cycles of adipocyte differentiation induction and maintenance. hMSCs were differentiated towards osteocytes with osteogenic differentiation medium for 3 days and for 7 days. ArrayCGH experiments were performed before differentiation induction, after 2 and 3 cycles of adipogenic differentiation induction and after 3 days and 7 days of osteogenic differentiation induction. Overall design: Genomic DNA was isolated form hMSCs before differentiation induction, after 2 and 3 cycles adipogenic differentiation induced cells and from 3-day and 7-day osteogenic differentiation induced cells.

INSTRUMENT(S): Agilent-021529 SurePrint G3 Human CGH Microarray Kit, 1x1M, design ID 21529 [hg19:GRCh37:Feb2009]

SUBMITTER: Valentina Galata 

PROVIDER: GSE88947 | GEO | 2016-10-20



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Gene amplification in mesenchymal stem cells and during differentiation towards adipocytes or osteoblasts.

Altmayer Nora Corinna NC   Galata Valentina V   Warschburger Nadine N   Keller Andreas A   Meese Eckart E   Fischer Ulrike U  

Oncotarget 20171201 2

Gene amplifications are an attribute of tumor cells and have for long time been overlooked in normal cells. A growing number of investigations describe gene amplifications in normal mammalian cells during development and differentiation. Possibly, tumor cells have rescued the gene amplification mechanism as a physiological attribute of stem cells. Here, we investigated human mesenchymal stem cells (hMSCs) for gene amplification using array-CGH, single cell fluorescence in situ hybridization and  ...[more]

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