Human iPSCs differentiated into SMCs

ABSTRACT: Human iPSCs were differentiated towards an induced-SMC (iSMC) phenotype in a 10-day protocol. Proteomics was performed throughout the entire differentiation time course to provide a robust, well-defined starting and ending cell population. Proteomics data verified iPSC differentiation to iSMCs. Proteomics comparison with primary human SMCs showed a high correlation with iSMCs. After iSMC differentiation, we initiated calcification in the iSMCs by culturing the cells in osteogenic media for 17 days.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Smooth Muscle Cell, Stem Cell

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Sasha Singh  

LAB HEAD: Elena Aikawa

PROVIDER: PXD032353 | Pride | 2022-10-14

REPOSITORIES: Pride