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Human iPSCs differentiated into SMCs

ABSTRACT: Human iPSCs were differentiated towards an induced-SMC (iSMC) phenotype in a 10-day protocol. Proteomics was performed throughout the entire differentiation time course to provide a robust, well-defined starting and ending cell population. Proteomics data verified iPSC differentiation to iSMCs. Proteomics comparison with primary human SMCs showed a high correlation with iSMCs. After iSMC differentiation, we initiated calcification in the iSMCs by culturing the cells in osteogenic media for 17 days.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Smooth Muscle Cell, Stem Cell

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Sasha Singh

LAB HEAD: Elena Aikawa

PROVIDER: PXD032353 | Pride | 2022-10-14

REPOSITORIES: Pride

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Induced pluripotent stem cell-derived smooth muscle cells to study cardiovascular calcification.

Atkins Samantha K SK Sonawane Abhijeet R AR Brouwhuis Romi R Barrientos Johana J Ha Anna A Rogers Maximillian M Tanaka Takeshi T Okui Takehito T Kuraoka Shiori S Singh Sasha A SA Aikawa Masanori M Aikawa Elena E

Frontiers in cardiovascular medicine 20220722

Cardiovascular calcification is the lead predictor of cardiovascular events and the top cause of morbidity and mortality worldwide. To date, only invasive surgical options are available to treat cardiovascular calcification despite the growing understanding of underlying pathological mechanisms. Key players in vascular calcification are vascular smooth muscle cells (SMCs), which transform into calcifying SMCs and secrete mineralizing extracellular vesicles that form microcalcifications, subseque ...[more]

PMID: 35958427

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