Transcriptomics,Genomics

Dataset Information

154

Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles


ABSTRACT: Dendritic cell subsets purified from skin-draining lymph nodes of C57BL/6J mice following intradermal injection of non-viable Nippostrongylus brasiliensis (Nb) or topical application of Dibutyl Phthalate-FITC. Results provide insight into the genetic programme induced in DC exposed to Th2 stimuli. Overall design: CD11b+, TN and CD103+ (Nb only) migratory DC subsets were flow sorted from skin-draining lymph nodes following treatment with Th2 stimuli or respective controls. Three independent replicates were generated for each DC population from pooled lymph nodes in three independent flow cytometry sorting experiments.

INSTRUMENT(S): Illumina HiSeq 2000 (Mus musculus)

SUBMITTER: Franca Ronchese  

PROVIDER: GSE88998 | GEO | 2016-10-20

SECONDARY ACCESSION(S): PRJNA349476

REPOSITORIES: GEO

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Publications


The dendritic cell signals required for the in vivo priming of IL-4-producing T cells are unknown. We used RNA sequencing to characterize DCs from skin LN of mice exposed to two different Th2 stimuli: the helminth parasite Nippostrongylus brasiliensis (Nb) and the contact sensitizer dibutyl phthalate (DBP)-FITC. Both Nb and DBP-FITC induced extensive transcriptional changes that involved multiple DC subsets. Surprisingly, these transcriptional changes were highly distinct in the two models, with  ...[more]

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