Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles

ABSTRACT: Dendritic cell subsets purified from skin-draining lymph nodes of C57BL/6J mice following intradermal injection of non-viable Nippostrongylus brasiliensis (Nb) or topical application of Dibutyl Phthalate-FITC. Results provide insight into the genetic programme induced in DC exposed to Th2 stimuli.

ORGANISM(S): Mus musculus

PROVIDER: GSE88998 | GEO | 2016/10/20

SECONDARY ACCESSION(S): PRJNA349476

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Neutrophils promote M2 polarization of macrophages during Nippostrongylus brasiliensis infection

Project description:Microarray profiling of amplified total RNA isolated from neutrophils sorted from naïve, Nippostrongylus brasiliensis (Nb)-infected, or lipopolysaccharide (LPS)-treated mice.

2014-08-30 | GSE46437 | GEO

Expression profiles of innate and Th2 lymphocytes

Project description:We compare the transcription profiles of IL-5-reporter marked ILC2s and Th2 cells sorted from mouse lung tissue after Nippostrongylus brasiliensis infection

2016-10-17 | GSE70020 | GEO

Single CD4+ cells after Nippostrongylus brasiliensis infection

Project description:We were interested in investigating the heterogeneity of CD4+ Th2 cells during infection response. In order to do so we injected Nippostrongylus brasiliensis (Nb) in recipient mice as this is known to elicit a Type 2 response. Mice were subcutaneously injected with Nb larvae and single CD4+ cells were isolated from mediastinal lymph nodes, mesenteric lymph nodes, and lungs 5 days after infection.

2016-04-13 | E-MTAB-4619 | biostudies-arrayexpress

miRNA data from extracellular vesicles secreted by Nippostrongylus brasiliensis

Project description:miRNA-Seq analysis of the miRNA present in the extracellular vesicles secreted by the nematode Nippostrongylus brasiliensis

2018-04-01 | GSE111478 | GEO

Th2 single-cell heterogeneity and clonal interorgan distribution in helminth-infected mice

Project description:Th2 cells provide effector functions in type 2 immune responses to helminths and allergens. Despite substantial knowledge about molecular mechanisms of Th2 cell differentiation, there is little information on Th2 cell heterogeneity and clonal distribution between organs mainly due to technical limitations. To address this issue, we performed combined single-cell transcriptome and TCR clonotype analysis on murine Th2 cells in mesenteric lymph nodes (MLN) and lung after infection with Nippostrongylus brasiliensis (Nb) as a model of human hookworm infection. We identified strong organ-specific expression profiles, but also found populations with conserved effector or migration signatures. A substantial MLN subpopulation with an interferon response signature suggests a role for interferon-signaling in Th2 cell differentiation or diversification. RNA-inferred developmental directions further implied proliferation as a hub for differentiation decisions. Although the TCR repertoire appeared to be highly heterogeneous, we identified expanded Th2 clones and CDR3 motifs. Clonal relatedness between distant organs confirmed the effective exchange of Th2 effector cells. However, locally expanded clones dominated the response, as the most expanded clones in MLN and lung did not overlap. This new insight in Th2 cell subsets and clonal relatedness in distant organs demonstrates their heterogeneity and suggests that they serve distinct effector functions.

2022-08-19 | GSE181342 | GEO

Extracellular vesicles from Nippostrongylus brasiliensis

Project description:We identified the extracellular vesicles (EVs)secreted by the nematode Nippostrongylus brasiliensis. EV proteins were analysed using a 5600+ mass spectrometer (ABSCIEX).

2018-10-19 | PXD009165 | Pride

Human resistin alters lung mRNA expression from helminth-infected lungs.

Project description:Goal: To examine the effects of human resistin during helminth infection. Methodology: To examine the function of human resistin (hResistin), we utilized transgenic mice expressing the human resistin gene along with its entire regulatory region (hRetnTg+). Following infection with the helminth Nippostrongylus brasiliensis, whole lung RNA was sequenced in hRetnTg+ mice, control hRetnTg- and naïve mice. Conclusion: In hRetnTg+ mice, many genes involved in inflammation and the immune system, specifically toll-like receptor signaling and chemokines, are significantly upregulated, suggesting that human resistin promotes TLR signaling and inflammation during helminth infection. Examination of whole lung mRNA from Nippostrongylus brasiliensis-infected lungs at day 7 in mice expressing human resistin

2014-11-14 | E-GEOD-60537 | biostudies-arrayexpress

Identification of Rat foreskin biomarkers of in utero dibutyl phthalate exposure [Illumina]

Project description:Dibutyl phthalate was administered to pregnant Sprague Dawley rats from gestational days 16-20 at either a 100 mg/kg/day or 500 mg/kg/day dose level. This timeframe covers the reproductive masculinization window which corresponds to increased androgen signalling. Dibutyl phthalate has been shown to disrupt testosterone production leading to male reproductive abnormalities. As such, we selected this exposure window for our study and examined gene expression changes in the male rat foreskin, which expresses the androgen receptor. We collected tissue samples at both gestational day 20 to identify gene expression changes immediately after exposure, and postnatal day 5 to identify gene expression changes persisting after birth using microarray analysis (Illumina RatRef 12 Bead Chips). To determine whether gene expression changes were brought on by decreased androgen signalling or additional effects of dibutyl phthalate exposure, we exposed rats to the potent androgen receptor antagonist flutamide (5 mg/kg/day) during the same period of development. Gene expression changes were compared to determine which were brought on by disruption of androgen signalling and which were the result of other aspects of chemical exposure. Two foreskin samples per litter were pooled for gene expression microarray analysis using the Illumina ratRef-12 v1.0 expression beadchip.

2013-07-12 | E-GEOD-48802 | biostudies-arrayexpress

Genome-Wide Transcriptomic Analysis of Intestinal Tissue to Assess the Impact of Nutrition and a Secondary Nematode Challenge in Lactating Rats

Project description:We use a genome wide transcriptomic approach to assess the effects of protein supplementation on gene expression in the small intestine of periparturient rats during a secondary infection with the rat nematode Nippostrongylus brasiliensis

2011-05-31 | E-TABM-1132 | biostudies-arrayexpress

Identification of Rat foreskin biomarkers of in utero dibutyl phthalate exposure [Affymetrix]

2013-06-04 | GSE47618 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data