Genomics

Dataset Information

151

A genome-wide CRISPR activation screen identifies B4GALNT2 as an inhibitory host factor for influenza virus infection


ABSTRACT: Identification of factors to inhibit influenza virus infection Overall design: CRISPR activation technology was used to identify genes that rendered the cell refractory to infection. The complete sgRNA library (plasmid sample) were packaged into lentivirus and used to transduce A549 cells (transduced sample). In three independent biological replicates, the transduced cells were infected with influenza virus, and the cells that were refractory to infection were collected and the sgRNAs enriched in those populations were sequenced (samples A, B, and C).

INSTRUMENT(S): Illumina HiSeq 2000 (Homo sapiens)

SUBMITTER: Nicholas Heaton  

PROVIDER: GSE89371 | GEO | 2017-07-17

SECONDARY ACCESSION(S): PRJNA351924

REPOSITORIES: GEO

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Publications

A CRISPR Activation Screen Identifies a Pan-avian Influenza Virus Inhibitory Host Factor.

Heaton Brook E BE   Kennedy Edward M EM   Dumm Rebekah E RE   Harding Alfred T AT   Sacco Matthew T MT   Sachs David D   Heaton Nicholas S NS  

Cell reports 20170801 7


Influenza A virus (IAV) is a pathogen that poses significant risks to human health. It is therefore critical to develop strategies to prevent influenza disease. Many loss-of-function screens have been performed to identify the host proteins required for viral infection. However, there has been no systematic screen to identify the host factors that, when overexpressed, are sufficient to prevent infection. In this study, we used CRISPR/dCas9 activation technology to perform a genome-wide overexpre  ...[more]

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