Transcriptomics,Genomics

Dataset Information

52

Expression data from D.melanogaster guts with a constitutively active Imd in the presence or absence of a microbiome


ABSTRACT: Innate immune responses contributed to the containment of intestinal microbes. We used microarrays to examine transcriptional profiles of imflamed guts raised in convential or germ-free conditions. We found that gut inflammation and the gut microbiome regulate the expression of several hundred host genes. Overall design: Genotype control flies and flies with a constitutively active Imd were raised on regular food or food supplemented with antibiotics to obtain conventionally reared (CR) or germ-free (GF) flies. Guts were dissected for RNA extraction and RNA was hybridized to Affymetrix GeneChip®Drosophila Genome 2.0 arrays to study the effects of gut inflammation in a microbiome dependant or independant manner on the transcriptional profile in host's gut.

INSTRUMENT(S): [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array

SUBMITTER: Edan Foley  

PROVIDER: GSE89445 | GEO | 2016-11-03

SECONDARY ACCESSION(S): PRJNA352228

REPOSITORIES: GEO

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Publications

Constitutive Immune Activity Promotes Tumorigenesis in Drosophila Intestinal Progenitor Cells.

Petkau Kristina K   Ferguson Meghan M   Guntermann Silvia S   Foley Edan E  

Cell reports 20170801 8


Gut innate immune defenses control bacterial populations and protect the host interior from invasion. Although excess intestinal immune activity frequently promotes inflammatory illnesses, we know little about the consequences of chronic innate immune activity exclusively in endodermal gut cells of an otherwise normal animal. To address this question, we examined the consequences of persistent inflammatory signals in adult fly intestinal progenitor cells. We found that constitutive immune activi  ...[more]

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