Dataset Information


Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells [rnaseq]

ABSTRACT: To follow the changes in the transcriptional programs accompanying the specification, maintenance and differentiation of the adult ISCs we sequenced whole transcriptomes of embryonic intestinal epithelium progenitors (at E12.5 and E14.5), adult ISCs and their differentiated progenies, the majority of which are absorptive enterocytes. EpCAM positive embryonic gut epithelium was isolated from dissected small intestines using fluorescence activated cell sorting (FACS). Adult ISCs were purified on the basis of GFP fluorescence from crypts of Lgr5GFP-Cre-ERT mice (Barker et al. 2007), whereas enterocytes (EpCAM+ CD45- CD31-) were isolated from villi. Overall design: Intestinal epithelial cells from two embryonic stages (E12.5 and E14.5), adult Lgr5+ ISCs and enterocytes were used. For each stage biological triplicates were analyzed.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Sergi Sayols 

PROVIDER: GSE89683 | GEO | 2017-03-29



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Dynamic changes in chromatin states during specification and differentiation of adult intestinal stem cells.

Kazakevych Juri J   Sayols Sergi S   Messner Berith B   Krienke Christina C   Soshnikova Natalia N  

Nucleic acids research 20170601 10

Epigenetic mechanisms, including chromatin structure, chromatin dynamics and histone modifications play an important role for maintenance and differentiation of pluripotent embryonic stem cells. However, little is known about the molecular mechanisms of adult stem cell specification and differentiation. Here, we used intestinal stem cells (ISCs) as a model system to reveal the epigenetic changes coordinating gene expression programs during these processes. We found that two distinct epigenetic m  ...[more]

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