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Id2 controls specification of Lgr5+ intestinal stem cell progenitors during gut development

ABSTRACT: To follow the changes in the transcriptional programs accompanying the specification of the adult ISCs we sequenced whole transcriptomes of embryonic intestinal epithelium progenitors (at E11.5 and E12.5) and adult ISCs. EpCAM positive embryonic gut epithelium was isolated from dissected small intestines using fluorescence activated cell sorting (FACS). Adult ISCs were purified on the basis of GFP fluorescence from crypts of Lgr5GFP-Cre-ERT mice (Barker et al. 2007) Double positive adlut ISCs were isolated by FACS based on GFP and tdTomato fluorescence.

ORGANISM(S): Mus musculus

PROVIDER: GSE90470 | GEO | 2017/01/11

SECONDARY ACCESSION(S): PRJNA354778

REPOSITORIES: GEO

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