Transcriptomics,Genomics

Dataset Information

159

Id2 controls specification of Lgr5+ intestinal stem cell progenitors during gut development


ABSTRACT: To follow the changes in the transcriptional programs accompanying the specification of the adult ISCs we sequenced whole transcriptomes of embryonic intestinal epithelium progenitors (at E11.5 and E12.5) and adult ISCs. EpCAM positive embryonic gut epithelium was isolated from dissected small intestines using fluorescence activated cell sorting (FACS). Adult ISCs were purified on the basis of GFP fluorescence from crypts of Lgr5GFP-Cre-ERT mice (Barker et al. 2007) Double positive adlut ISCs were isolated by FACS based on GFP and tdTomato fluorescence. Overall design: Intestinal epithelial cells from two embryonic stages (E11.5 and E13.5), mesenchymal (E11.5) and adult Lgr5+ ISCs were used. For embryonic stages biological triplicates were analysed. For the adult ISCs either 4 biological replicates ot duplicates were analysed.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Sergi Sayols 

PROVIDER: GSE90470 | GEO | 2017-01-11

SECONDARY ACCESSION(S): PRJNA354778

REPOSITORIES: GEO

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Publications

Id2 controls specification of Lgr5+ intestinal stem cell progenitors during gut development.

Nigmatullina Lira L   Norkin Maxim M   Dzama Margarita M MM   Messner Berith B   Sayols Sergi S   Soshnikova Natalia N  

The EMBO journal 20170111 7


The adult intestinal stem cells (ISCs), their hierarchies, mechanisms of maintenance and differentiation have been extensively studied. However, when and how ISCs are established during embryogenesis remains unknown. We show here that the transcription regulator Id2 controls the specification of embryonic Lgr5+ progenitors in the developing murine small intestine. Cell fate mapping analysis revealed that Lgr5+ progenitors emerge at E13.5 in wild-type embryos and differ from the rest on the intes  ...[more]

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