Genomics

Dataset Information

37

Integration host factor and LuxR synergistically bind DNA to coactivate quorum-sensing genes in Vibrio harveyi.


ABSTRACT: LuxR is the master quorum sensing regulator in Vibrio harveyi. This transcription factor controls the expression of over 600 genes to coordinate group behaviors. In order to understand how LuxR regulates transcription we identified a co-regulator, integration host factor (IHF). In this study we performed RNA-seq to show that the LuxR and IHF regulons overlap significantly. Overall design: Wild-type, ΔluxR, ΔihfA ΔihfB strains were grown at 30 °C to OD600 = 1.0. RNA was extracted and prepared from three biological replicates of each strain. cDNA libraries were created and sequenced for differential gene expression analysis.

INSTRUMENT(S): Illumina NextSeq 500 (Vibrio harveyi)

ORGANISM(S): Vibrio Harveyi

SUBMITTER: Julia C van Kessel 

PROVIDER: GSE90845 | GEO | 2017-12-03

SECONDARY ACCESSION(S): PRJNA356167

REPOSITORIES: GEO

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Publications

Integration host factor and LuxR synergistically bind DNA to coactivate quorum-sensing genes in Vibrio harveyi.

Chaparian Ryan R RR   Olney Stephen G SG   Hustmyer Christine M CM   Rowe-Magnus Dean A DA   van Kessel Julia C JC  

Molecular microbiology 20160616 5


The cell-cell signaling process called quorum sensing allows bacteria to control behaviors in response to changes in population density. In Vibrio harveyi, the master quorum-sensing transcription factor LuxR is a member of the TetR family of transcription factors that both activates and represses genes to coordinate group behaviors, including bioluminescence. Here, we show that integration host factor (IHF) is a key coactivator of the luxCDABE bioluminescence genes that is required together with  ...[more]

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