Genomics

Dataset Information

30

DNA methylation profiling in lungs tissues of smokers and patients with COPD


ABSTRACT: Epigenetics changes have been shown to be affected by cigarette smoking. It is possible that cigarette smoke (CS)-mediated DNA methylation would affect several cellular and pathophysiological processes, acute exacerbations, and comorbidity in lungs of patients with chronic obstructive pulmonary disease (COPD). We sought to determine whether genome-wide lung DNA methylation profiles of smokers and patients with COPD were significantly different from non-smokers. We isolated DNA from lung tissues of patients including 8 lifelong non-smokers, 8 current smokers, and 8 patients with COPD, and subsequently analyzed the samples using the Illumina’s Infinium HumanMethylation450 BeadChip. Overall design: We performed Genome-Wide DNA methylation analysis using lung parenchyma tissue DNA samples from Non-smokers (NS), Smokers (S) and patients with COPD (C) in 2 separate batches. In the first batch of samples, we used Sample ID# 1-4 in each of the three groups: Non-smokers (NS.1, NS.2, NS.3, NS.4), Smokers (S.1, S.2, S.3, S.4) and COPD (C.1, C.2, C.3, C.4). The second batch of samples, we used additional samples in each of the three groups (Sample ID# 5-8): Non-smokers (NS.5, NS.6, NS.7, NS.8), Smokers (S.5, S.6, S.7, S.8) and patients with COPD (C.5, C.6, C.7, C.8).

INSTRUMENT(S): Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)

SUBMITTER: Isaac Kirubakaran Sundar  

PROVIDER: GSE92511 | GEO | 2017-06-12

SECONDARY ACCESSION(S): PRJNA357831

REPOSITORIES: GEO

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Publications

DNA methylation profiling in peripheral lung tissues of smokers and patients with COPD.

Sundar Isaac K IK   Yin Qiangzong Q   Baier Brian S BS   Yan Li L   Mazur Witold W   Li Dongmei D   Susiarjo Martha M   Rahman Irfan I  

Clinical epigenetics 20170414


BACKGROUND:Epigenetics changes have been shown to be affected by cigarette smoking. Cigarette smoke (CS)-mediated DNA methylation can potentially affect several cellular and pathophysiological processes, acute exacerbations, and comorbidity in the lungs of patients with chronic obstructive pulmonary disease (COPD). We sought to determine whether genome-wide lung DNA methylation profiles of smokers and patients with COPD were significantly different from non-smokers. We isolated DNA from parenchy  ...[more]

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