Transcriptomics,Genomics

Dataset Information

92

Early ETI and PTI responses in Arabidopsis thaliana camta3-D (sr1-4d) mutant


ABSTRACT: Innate immune responses of plant cells confer the first line of defence against pathogens. Signals generated by activated receptors are integrated inside the cell and converge on transcriptional programmes in the nucleus. In Arabidopsis, the CAMTA family of transcription factors plays a pivotal function in immunity. CAMTA binding motifs are highly enriched in the genes quickly induced during ETI and PTI. Using RNA-seq, we investigated the role of CAMTA TFs during the early ETI and PTI transcriptional responses. Overall design: We compared the expression changes between an Arabidopsis mutant line carrying the camta3-D (sr1-4d) dominant negative mutation and Col-0 wild-type plants following treatment with the PAMP flg22 (100 nM), and the avirulent bacterial strains Pst DC3000 AvrRpm1 (OD600=0.001) and Pst DC3000 AvrRps4 (OD600=0.001). The expression changes were analysed at one time point after each treatment (1 hour for flg22 and 4 hours for Pst DC3000 AvrRpm1 and Pst DC3000 AvrRps4 treatments).

INSTRUMENT(S): Illumina HiSeq 2500 (Arabidopsis thaliana)

SUBMITTER: Barbara Kracher  

PROVIDER: GSE92702 | GEO | 2017-12-11

SECONDARY ACCESSION(S): PRJNA358395

REPOSITORIES: GEO

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Publications

A dominant-interfering camta3 mutation compromises primary transcriptional outputs mediated by both cell surface and intracellular immune receptors in Arabidopsis thaliana.

Jacob Florence F   Kracher Barbara B   Mine Akira A   Seyfferth Carolin C   Blanvillain-Baufumé Servane S   Parker Jane E JE   Tsuda Kenichi K   Schulze-Lefert Paul P   Maekawa Takaki T  

The New phytologist 20171211 4


Pattern recognition receptors (PRRs) and nucleotide-binding domain and leucine-rich repeat (LRR)-containing proteins (NLRs) initiate pattern-triggered immunity (PTI) and effector-triggered immunity (ETI), respectively, each associated with the activation of an overlapping set of defence genes. The regulatory mechanism behind this convergence of PTI- and ETI-mediated defence gene induction remains elusive. We generated transgenic Arabidopsis plants that enable conditional NLR activation without p  ...[more]

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