Genomics

Dataset Information

43

β2→1-fructans modulate the immune system in vivo in a microbiota-dependent and -independent fashion


ABSTRACT: It has been shown in vitro that only specific dietary-fibers contribute to immunity but studies in vivo are not conclusive. Here we investigated degree of polymerization (DP) dependent effects of β2→1-fructans on immunity via microbiota-dependent and -independent effects. To this end, conventional or germ-free mice received short- or long-chain β2→1-fructan for 5 days. Immune cell populations in the spleen, mesenteric lymph nodes (MLN), and Peyer's patches (PPs) were analyzed with flow cytometry, genome-wide gene expression in the ileum was measured with microarray, and gut microbiota composition was analyzed with 16S rRNA sequencing of fecal samples. We found that β2→1-fructans modulated immunity by both microbiota and microbiota-independent effects. Moreover, effects were dependent on the chain-length of the β2→1-fructans type polymer. Both short- and long-chain β2→1-fructans enhanced T-helper 1 cells in Peyer's patches, whereas only short-chain β2→1-fructans increased regulatory T cells and CD11b-CD103- DCs in the MLN. A common feature after short- and long-chain β2→1-fructan treatment was enhanced Fut2 expression and other IL-22-dependent genes in the ileum of conventional mice. These effects were not associated with shifts in gut microbiota composition, or altered production of short-chain fatty acids. Both short- and long-chain β2→1-fructans also induced immune effects in germ-free animals, demonstrating direct effect independent from the gut microbiota. Also, these effects were dependent on the chain-length of the β2→1-fructans. Short-chain β2→1-fructan induced lower CD80 expression by CD11b-CD103- DCs in PPs, whereas long-chain β2→1-fructan specifically modulated B cell responses in germ-free mice. In conclusion, support of immunity is determined by the chemical structure of β2→1-fructans and is partially microbiota-independent. Overall design: Conventional or germ-free mice received short-chain (oligofructose; FOS) or long-chain (inulin; IN) β2→1-fructan for 5 days. Genome-wide gene expression in the ileum was measured with microarray.

INSTRUMENT(S): [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]

SUBMITTER: Guido Hooiveld  

PROVIDER: GSE94516 | GEO | 2018-03-22

REPOSITORIES: GEO

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It has been shown <i>in vitro</i> that only specific dietary fibers contribute to immunity, but studies <i>in vivo</i> are not conclusive. Here, we investigated degree of polymerization (DP) dependent effects of β2→1-fructans on immunity <i>via</i> microbiota-dependent and -independent effects. To this end, conventional or germ-free mice received short- or long-chain β2→1-fructan for 5 days. Immune cell populations in the spleen, mesenteric lymph nodes (MLNs), and Peyer's patches (PPs) were anal  ...[more]

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