Dataset Information


Next Generation Sequencing Facilitates Quantitative Analysis of FoxO3 or Geminin/FoxO3 depletion on MDA-MB-231 cells Transcriptomes

ABSTRACT: We generated 2 Gb of high-quality sequencing data (~1 Gb per sample) and catalogued the expression profiles of 48,162 annotated human genes in each sample. The analysis showed differences of transcriptomes between Control and Geminin/FoxO3 co-depletion expression changes. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms by Geminin and FoxO3 . Overall design: MDA-MB-231 mRNA profiles of Control, FoxO3 and Geminin/FoxO3 co-depletion were generated by deep sequencing, using Illumina HiSeq4000.

INSTRUMENT(S): Illumina HiSeq 4000 (Homo sapiens)

SUBMITTER: Lei Zhang  

PROVIDER: GSE94687 | GEO | 2017-08-22



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Geminin facilitates FoxO3 deacetylation to promote breast cancer cell metastasis.

Zhang Lei L   Cai Meizhen M   Gong Zhicheng Z   Zhang Bingchang B   Li Yuanpei Y   Guan Li L   Hou Xiaonan X   Li Qing Q   Liu Gang G   Xue Zengfu Z   Yang Muh-Hua MH   Ye Jing J   Chin Y Eugene YE   You Han H  

The Journal of clinical investigation 20170424 6

Geminin expression is essential for embryonic development and the maintenance of chromosomal integrity. In spite of this protective role, geminin is also frequently overexpressed in human cancers and the molecular mechanisms underlying its role in tumor progression remain unclear. The histone deacetylase HDAC3 modulates transcription factors to activate or suppress transcription. Little is known about how HDAC3 specifies substrates for modulation among highly homologous transcription factor fami  ...[more]

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