Transcriptomics,Genomics

Dataset Information

37

Expression data from HIF1A WT vs KD Madin-Darby canine kidney epithelial cells (MDCK strain II cells (ATCC:CCL-34)) under normoxic and hypoxic conditions


ABSTRACT: The expression of TGFbeta pathway genes were downregulated in HIF1A KD Madin-Darby canine kidney epithelial cells (MDCKII) in hypoxic condition. We used microarray to study the differential expression of genes in HIF1A knockdown versus WT Madin-Darby canine kidney epithelial cells (MDCKII) Overall design: Madin-Darby canine kidney epithelial cells (MDCKII) were incubated 24h in normoxic condition then additionally were incubated 24h in normoxic or hypoxic conditions and total RNA was extracted for microarray analysis.

INSTRUMENT(S): [Canine_2] Affymetrix Canine Genome 2.0 Array

SUBMITTER: Irina Raykhel  

PROVIDER: GSE94772 | GEO | 2017-02-11

SECONDARY ACCESSION(S): PRJNA374012

REPOSITORIES: GEO

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Publications

BAMBI is a novel HIF1-dependent modulator of TGFβ-mediated disruption of cell polarity during hypoxia.

Raykhel Irina I   Moafi Fazeh F   Myllymäki Satu M SM   Greciano Patricia G PG   Matlin Karl S KS   Moyano Jose V JV   Manninen Aki A   Myllyharju Johanna J  

Journal of cell science 20180523 10


Hypoxia and loss of cell polarity are common features of malignant carcinomas. Hypoxia-inducible factor 1 (HIF1) is the major regulator of cellular hypoxia response and mediates the activation of ∼300 genes. Increased HIF1 signaling is known to be associated with epithelial-mesenchymal transformation. Here, we report that hypoxia disrupts polarized epithelial morphogenesis of MDCK cells in a HIF1α-dependent manner by modulating the transforming growth factor-β (TGFβ) signaling pathway. Analysis  ...[more]

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