Genomics

Dataset Information

158

Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism


ABSTRACT: Vertical sleeve gastrectomy (VSG) produces sustainable weight loss, remission of type 2 diabetes (T2D), and improvement of nonalcoholic fatty liver disease (NAFLD). However, the molecular mechanisms underlying the metabolic benefits of VSG have remained elusive. We have previously demonstrated that diet-induced obesity leads to chromatin modifications in the liver of mice. We demonstrate here that VSG in C57BL/6J wild-type male mice can reverse these chromatin modifications and thereby impact the expression of key metabolic genes. Genes involved in lipid metabolism, especially omega-6 fatty acid metabolism, are up-regulated in livers of mice after VSG while genes in inflammatory pathways are down-regulated after VSG. Consistent with gene expression changes, regulatory regions near genes involved in inflammatory response displayed decreased chromatin accessibility after VSG. Our results indicate that VSG induces global regulatory changes that impact hepatic inflammatory and lipid metabolic pathways, providing new insight into the mechanisms underlying the beneficial metabolic effects induced by VSG. Overall design: Chromatin accessibility and transcriptome profiles of VSG, Sham and Control mice liver in duplicates.

INSTRUMENT(S): Illumina HiSeq 2500 (Mus musculus)

SUBMITTER: Juan Du 

PROVIDER: GSE94842 | GEO | 2017-07-13

SECONDARY ACCESSION(S): PRJNA374559

REPOSITORIES: GEO

altmetric image

Publications

Vertical sleeve gastrectomy reverses diet-induced gene-regulatory changes impacting lipid metabolism.

Du Juan J   Tian Jingyan J   Ding Lili L   Trac Candi C   Xia Brian B   Sun Siming S   Schones Dustin E DE   Huang Wendong W  

Scientific reports 20170713 1


Vertical sleeve gastrectomy (VSG) produces sustainable weight loss, remission of type 2 diabetes (T2D), and improvement of nonalcoholic fatty liver disease (NAFLD). However, the molecular mechanisms underlying the metabolic benefits of VSG have remained elusive. According to our previous results, diet-induced obesity induces epigenetic modifications to chromatin in mouse liver. We demonstrate here that VSG in C57BL/6J wild-type male mice can reverse these chromatin modifications and thereby impa  ...[more]

Similar Datasets

| GSE104956 | GEO
2014-03-27 | E-GEOD-53782 | ArrayExpress
| GSE79423 | GEO
| GSE110747 | GEO
| GSE95489 | GEO
2010-02-05 | E-GEOD-18745 | ArrayExpress
2010-01-28 | GSE18745 | GEO
2011-03-11 | E-GEOD-25937 | ArrayExpress
2011-03-11 | GSE25937 | GEO
| GSE121489 | GEO