Genomics

Dataset Information

36

Generation of mature T cells from human hematopoietic stem/progenitor cells in artificial thymic organoids


ABSTRACT: Mechanistic studies of human T cell development require robust model systems that recapitulate the full span of thymopoiesis, from hematopoietic stem and progenitor cells (HSPC) through to mature naïve T cells. We developed a serum free, artificial thymic organoid (ATO) system that supports highly efficient and reproducible in vitro differentiation and positive selection to generate conventional human T cells from all sources of HSPCs. Deep-sequencing of the TCR variable genes was performed to compare the diversity of the TCR repertoire between ATO-derived CD8SP T cells and naive CD8SP T cells from thymus and blood. Overall design: TCR repertoire analysis of CD3+TCRab+CD8SP T cells derived from cord blood CD34+CD3- HSPCs in ATOs compared with human naïve CD8SP T cells from the thymus and blood

INSTRUMENT(S): Illumina MiSeq (Homo sapiens)

SUBMITTER: Michael T Bethune 

PROVIDER: GSE94968 | GEO | 2017-02-17

SECONDARY ACCESSION(S): PRJNA374973

REPOSITORIES: GEO

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Publications

Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids.

Seet Christopher S CS   He Chongbin C   Bethune Michael T MT   Li Suwen S   Chick Brent B   Gschweng Eric H EH   Zhu Yuhua Y   Kim Kenneth K   Kohn Donald B DB   Baltimore David D   Crooks Gay M GM   Montel-Hagen Amélie A  

Nature methods 20170403 5


Studies of human T cell development require robust model systems that recapitulate the full span of thymopoiesis, from hematopoietic stem and progenitor cells (HSPCs) through to mature T cells. Existing in vitro models induce T cell commitment from human HSPCs; however, differentiation into mature CD3<sup>+</sup>TCR-αβ<sup>+</sup> single-positive CD8<sup>+</sup> or CD4<sup>+</sup> cells is limited. We describe here a serum-free, artificial thymic organoid (ATO) system that supports efficient and  ...[more]

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