Transcriptomics,Genomics

Dataset Information

38

Temporal changes in the rainbow darter (Etheostome caeruleum) liver transcriptome associated with sewage treatment plant upgrades in the Grand River, Ontario


ABSTRACT: In this study, adult male rainbow darter were collected from three sites in the Grand River in the fall season for four consecutive years (2011-2014), from 3 sites: a reference site (US2) located in the non-urbanized area, and two exposure sites (DSW and DSK1) located dowstrem of two secondary wastewater treatment palnts (WWTP) (Waterloo and Kitchener). Kitchener WWTP has been undergone infrastructure upgrades that were initiated in the summer of 2012. The goal of this project was to see whether the implemented upgrades can be reflected in the liver transcriptome response indicative of recovery.The RNA from male rainbow darter was exctracted from liver tissue and used for further gene expression analysis. Overall design: There were 86 samples processed, 6-7 samples per site. Fish were collected in 2011, 2012, 2013 and 2014 dowstream of Waterloo WWTP (DSW) and Kitchener WWTP (DSK1) representing exposure sites; US2 males were used as controls and all the comparisons in gene expression were done using fish from this site, using a one way-ANOVA within each year. There was a total of 79 microarrays subjected to bioniformatics analysis (7 arrays did not pass the quality standards).

INSTRUMENT(S): Agilent-042064 UNBSJ rainbow darter 15k v2.0

SUBMITTER: Patricija Marjan  

PROVIDER: GSE95083 | GEO | 2017-02-21

SECONDARY ACCESSION(S): PRJNA376009

REPOSITORIES: GEO

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Publications

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater-treatment plant upgrades.

Marjan Patricija P   Martyniuk Christopher J CJ   Fuzzen Meghan L M MLM   MacLatchy Deborah L DL   McMaster Mark E ME   Servos Mark R MR  

Environmental toxicology and chemistry 20170223 8


The present study measured hepatic transcriptome responses in male rainbow darter (Etheostoma caeruleum) exposed to 2 municipal wastewater-treatment plants (MWWTPs; Kitchener and Waterloo) over 4 fall seasons (2011-2014) in the Grand River (Ontario, Canada). The overall goal was to determine if upgrades at the Kitchener MWWTP (in 2012) resulted in transcriptome responses indicative of improved effluent quality. The number of differentially expressed probes in fish downstream of the Kitchener out  ...[more]

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