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Global miRNA expression profile in cardiac progenitor cells generated by ISX-9

ABSTRACT: We developed a strategy to generate cardiac progenitor cells from human induced pluripotent stem cells using a novel small molecule.miRNA-sequencing results showed different miRNA expression profile among undifferentiated human induced pluripotent stem cells(hiPSCs), DMSO and ISX-9 treated hiPSCs.In comparsion with DMSO treated cells, ISX-9 upregulated several myogenic miRNAs and cardiac hypertrophy related-miRNAs including miR-335, miR-21, miR-30c,miRNA-181a and miR-214.

ORGANISM(S): Homo sapiens

PROVIDER: GSE95390 | GEO | 2017/12/30

SECONDARY ACCESSION(S): PRJNA376872

REPOSITORIES: GEO

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Global mRNA expression profile in cardiac progenitor cells generated by ISX-9

Project description:We developed a strategy to generate cardiac progenitor cells from human induced pluripotent stem cells using a novel small molecule. mRNA-sequencing results showed different gene expression profile among undifferentiated human induced pluripotent stem cells(hiPSCs), DMSO and ISX-9 treated hiPSCs.In comparsion with DMSO treated cells or undifferentiated hiPSCs, ISX-9 upregulated the genes related to WNT and cytoskeleton remodeling and TGF-β signaling, which are involved in heart development and cardiac differentiation. In addition, the genes related to cardiac differentiation signaling pathways were upregulated by ISX-9 including development of PIP3 signaling in cardiomyocyte myocytes, muscle contraction and NF-AT hypertrophy signaling.

2017-12-30 | GSE95389 | GEO

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Project description:MCF7 cells were treated with ISX, a novel GLI1 inducing HDAC-inhibitor

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Transcriptional changes in IMR32 cells upon ISX treatment

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2021-04-28 | E-MTAB-10249 | biostudies-arrayexpress

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2023-10-05 | GSE242861 | GEO

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