Transcriptomics,Genomics

Dataset Information

155

NtrC-dependent control of exopolysaccharide synthesis and motility in Burkholderia cenocepacia H111


ABSTRACT: Burkholderia cenocepacia is a versatile opportunistic pathogen that survives in a wide variety of environments, which can be limited in nutrients such as nitrogen. We previously showed that B. cenocepacia sigma factor s54 played a major role in control of nitrogen assimilation and virulence. In this work, we investigated the role of the s54 enhancer binding protein NtrC in controlling the response to nitrogen limitation and virulence. RNA-Seq analyses and phenotypical analysis on a ntrC mutant strain showed that, in addition to orchestrating uptake of nitrogen sources, NtrC is also regulating exopolysaccharide (EPS) production and motility. A search for NtrC consensus sequences identified a potential binding sequence in the promoter region of gene clusters involved in EPS formation and flagellar rotation suggesting that NtrC directly controls the expression of these phenotypic traits in B. cenocepacia H111. Overall design: NtrC regulon in B. cenocepacia

INSTRUMENT(S): Illumina HiSeq 2500 (Burkholderia cenocepacia H111)

SUBMITTER: Gabriella Pessi  

PROVIDER: GSE95607 | GEO | 2017-08-17

SECONDARY ACCESSION(S): PRJNA377636

REPOSITORIES: GEO

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Publications

NtrC-dependent control of exopolysaccharide synthesis and motility in Burkholderia cenocepacia H111.

Liu Yilei Y   Lardi Martina M   Pedrioli Alessandro A   Eberl Leo L   Pessi Gabriella G  

PloS one 20170629 6


Burkholderia cenocepacia is a versatile opportunistic pathogen that survives in a wide variety of environments, which can be limited in nutrients such as nitrogen. We have previously shown that the sigma factor σ54 is involved in the control of nitrogen assimilation and virulence in B. cenocepacia H111. In this work, we investigated the role of the σ54 enhancer binding protein NtrC in response to nitrogen limitation and in the pathogenicity of H111. Of 95 alternative nitrogen sources tested the  ...[more]

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